FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5697203
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT403750.7086810843847411No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT125400.2201080073853784No Hit
GGGGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG93410.16395764728762519No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA88930.15609413952776477No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT64390.11302037157531512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT129550.088.7520759
GTCTACG15300.076.2850654
GGGGGAT135150.065.2685241
TACGGCC18350.063.6049737
GCCGGTT22650.063.372683
CGGTTCA22200.063.02455
GGGGGAA210800.062.2512741
CGGGGAT42850.059.349671
CGGGTAT14200.057.821751
CTTAGCG22900.057.3001569
CGGCATT21800.056.995521
CCGGGCG203200.056.9395835
GGGGGGA311400.056.3507421
CGGCAAT14650.056.0456541
GGTCTAC22100.054.4528853
GGCGCGG214850.054.190958
CGGCAAA26550.053.9141541
GGGGTAC35900.052.623541
CGGGGTA14200.051.169691
TCTACGG23200.050.6211625
Produced by FastQC (version 0.11.5)