FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006135211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6329476
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1963003.1013625772496805RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1833412.896622090043473RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA1654442.613865665972981RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1416812.238431743796801RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1297002.0491427726402627RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA1212041.9149136516198182RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA895241.414398285102906RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG855371.3514072886918285RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA832111.3146585910113255No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA769291.2154086689008696RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC685411.082885850266278RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG499610.7893386435148818RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC484480.7654346110167729RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC463960.7330148656855638RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC452410.7147669096146347RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG448190.7080996910328754RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA424240.6702608557169661RNA PCR Primer, Index 1 (100% over 32bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA389720.6157223757543279RNA PCR Primer, Index 1 (100% over 34bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG387080.6115514143666869Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA383990.6066694936516072RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA360780.5699997914519306RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC330730.5225235074751844RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA316230.49961481803548985RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA292900.4627555266818296RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA278240.4395940517034901RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA261500.4131463647227669RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA238360.3765872561962475RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC237740.37560771223399847RNA PCR Primer, Index 1 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA236100.3730166604628883RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC231070.365069715091739RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT226370.3576441398940449RNA PCR Primer, Index 8 (100% over 36bp)
AACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC221990.3507241357736407RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC218560.3453050457889405RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA218500.3452102512119487RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC210720.3329185543953401RNA PCR Primer, Index 1 (100% over 31bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG207690.3281314282572523Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG206400.3260933448519277RNA PCR Primer, Index 1 (100% over 29bp)
TAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGT204470.32304411929202353RNA PCR Primer, Index 1 (100% over 30bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG201840.3188889570005479Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA194240.3068816439149149RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA190650.3012097683915698RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG175400.2771161467394773RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC172560.27262920342853025RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC168270.2658513911736137RNA PCR Primer, Index 1 (100% over 31bp)
ATCCCACTTCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA167570.2647454544420423RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC166170.26253358097889934RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC159290.25166380281716844RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG154990.2448701914660866RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG153000.2417261713291906RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA151560.23945110148138643RNA PCR Primer, Index 1 (100% over 28bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC150670.23804498192267418RNA PCR Primer, Index 1 (100% over 33bp)
TAGCAGCACATAATGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT147460.23297347205361077RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG143720.2270646100877861RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA139410.22025519964053897RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC137780.217679946965594RNA PCR Primer, Index 1 (100% over 33bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA132070.20865866305520395RNA PCR Primer, Index 1 (100% over 34bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA130650.2064151913997304RNA PCR Primer, Index 1 (100% over 32bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA124970.19744130477783628RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC119250.1884042217712809RNA PCR Primer, Index 1 (100% over 27bp)
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG116440.18396467574882977Illumina Small RNA Adapter 2 (100% over 21bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC114430.18078905741960313RNA PCR Primer, Index 5 (100% over 35bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG112400.1775818408980459RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG112330.17747124722488877RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC111930.1768392833782765RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC111600.17631791320482137RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC110820.17508558370392746RNA PCR Primer, Index 5 (100% over 35bp)
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT110640.17480119997295196RNA PCR Primer, Index 1 (100% over 30bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGA109980.1737584596260417RNA PCR Primer, Index 8 (100% over 39bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG108580.1715465861628988No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA106950.16897133348795382RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA106760.168671150660813RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC103740.1638998236188904RNA PCR Primer, Index 1 (100% over 33bp)
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT101030.15961826855809233RNA PCR Primer, Index 1 (100% over 30bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC100860.15934968392328214RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA98140.15505232976631872RNA PCR Primer, Index 1 (100% over 32bp)
TTACTTGATGACAATAAAATATCTGATTGGAATTCTCGGGTGCCAAGGAA98010.15484694151616973RNA PCR Primer, Index 1 (100% over 23bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC96200.15198730511024927RNA PCR Primer, Index 1 (100% over 27bp)
TCAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAAC92240.14573086302878785RNA PCR Primer, Index 1 (100% over 24bp)
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA91030.14381917239278574RNA PCR Primer, Index 1 (100% over 23bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT89700.1417178926028RNA PCR Primer, Index 8 (100% over 36bp)
CCCATAAAGTAGAAAGCACTAATGGAATTCTCGGGTGCCAAGGAACTCCA89440.14130711610250202RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG89070.14072254954438568RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT88700.1401379829862693RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC87550.13832108692725908RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA87220.13779971675380392RNA PCR Primer, Index 1 (100% over 28bp)
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT87120.1376417257921509RNA PCR Primer, Index 8 (100% over 36bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA85290.13475049119389979RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA84920.13416592463578345RNA PCR Primer, Index 1 (100% over 32bp)
TTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAG84760.13391313909713853RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC84290.13317058157736913RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC84130.13291779603872422RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC83820.13242802405759974RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG82710.1306743243832507No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA81500.12876263374724858RNA PCR Primer, Index 1 (100% over 22bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA80960.12790948255432202RNA PCR Primer, Index 1 (100% over 22bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG80300.12686674220741181RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTT79110.12498664976374031RNA PCR Primer, Index 8 (100% over 37bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG78570.12413349857081377Illumina Small RNA Adapter 2 (100% over 21bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT78400.12386491393600356RNA PCR Primer, Index 1 (100% over 30bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA78220.12358053020502804RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG75550.11936217152889118RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG74530.1177506637200299RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA74190.11721349445040948RNA PCR Primer, Index 1 (100% over 28bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC72470.11449604990997676No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC72040.11381668877486857RNA PCR Primer, Index 5 (100% over 35bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA71660.11321632312058692RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA71520.11299513577427261RNA PCR Primer, Index 1 (100% over 32bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC71240.11255276108164405RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG70180.11087805688812155RNA PCR Primer, Index 1 (100% over 29bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC70130.11079906140729501RNA PCR Primer, Index 1 (100% over 33bp)
CATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC68990.10899796444445006RNA PCR Primer, Index 1 (100% over 31bp)
ATCCCATCCTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA67410.10650170725033162RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC67090.10599613617304181RNA PCR Primer, Index 1 (100% over 27bp)
CATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT66580.10519038226861119RNA PCR Primer, Index 1 (100% over 30bp)
CCCATAAAGTAGAAAGCACTATTGGAATTCTCGGGTGCCAAGGAACTCCA66380.10487440034530504RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG65990.1042582355948581Illumina Small RNA Adapter 2 (100% over 21bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT65750.10387905728689074RNA PCR Primer, Index 1 (100% over 30bp)
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG65150.10293111151697233Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA64020.10114581365029271No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG207.917998E-570.0238651
CTATACG750.070.023861
GCCATAA150.002222363470.023861
CCCCCTA252.8367267E-670.023861
CGACGAA150.002225917869.995653
GTGTACA150.002225987669.99509412
CTTACGG150.002225987669.99509410
TCAACGC208100.069.793846
TATTGCA288600.069.7569661
CAACGCT208550.069.742827
GTACCAG309250.069.733714
TGAGGTA407100.069.722851
TACCAGT307600.069.6987315
TCCCAAC309750.069.679283
GGTAGTA418250.069.677674
GAGGTAG418600.069.661772
CTTGTAC311500.069.64680511
ATTCAAC208800.069.6436544
TTCAACG208400.069.642985
CATCCTA29550.069.639247