FastQCFastQC Report
Thu 5 Jan 2023
EGAF00006135431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40129350
Sequences flagged as poor quality0
Sequence length32-97
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC1854010.4620084800775492No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC779800.19432161248562463No Hit
CTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCAC755310.18821884730253544No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA743260.18521605757382065No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA710260.17699265001800427No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG637650.15889866145352466No Hit
AGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAG580520.144662198615228No Hit
GGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGG576360.14362555087485843No Hit
GGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG561190.13984527534086647No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC546380.13615470970748345No Hit
TCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGAT430360.1072432022945799No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG412400.10276767503086892No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA410290.10224187533563339No Hit
AGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGA409790.10211727825145435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)