FastQCFastQC Report
Thu 5 Jan 2023
EGAF00006135449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31316732
Sequences flagged as poor quality0
Sequence length32-97
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC1313380.4193860329998673No Hit
CTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCAC579250.18496502125445274No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC541050.1727670690543317No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG528440.1687404675558101No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA474670.15157073222071832No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC473070.15105982322804307No Hit
AGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAG462800.14778042613130898No Hit
GGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGG444400.1419049727155439No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG433010.1382679393239371No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG409320.13070329305113956No Hit
CTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGCGCGGC407070.12998482728019004No Hit
AGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGA386090.12328553311373613No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA372280.11887574987070809No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA365950.11685446616843673No Hit
ACACGACCGGTCGGAGGCAGAACGGCAGCCCCTCGGCGGCCGGCCGGCGC348510.11128555814827679No Hit
GCAACACGCCCCCACCGCCGACGACGCGCGACGACGACGACGACGGGCAC317370.10134199187833519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)