FastQCFastQC Report
Thu 5 Jan 2023
EGAF00006135450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31316732
Sequences flagged as poor quality0
Sequence length32-97
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT629800.2011065522417856No Hit
AGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCAC494500.15790281054868688No Hit
GCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGG466960.14910878951226456No Hit
AATCGCCCCCGGGGGTGCCTCCGGGCTCCTCGGGGTGCGCGGCTGGGGGT444230.14185068863507214No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC404730.1292376228784025No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG400260.1278102708801161No Hit
TGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAG359740.11487150064061602No Hit
AGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCAC325110.10381351413040159No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC319760.10210516218614382No Hit
TGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGC317540.10139627595880694No Hit
AGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCG314790.10051815112764641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)