FastQCFastQC Report
Thu 5 Jan 2023
EGAF00006135495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006135495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35190873
Sequences flagged as poor quality0
Sequence length32-97
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC1144350.3251837486384609No Hit
TCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCG850210.2415995761173643No Hit
CTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGCGCGGC712130.20236212952148133No Hit
GCAACACGCCCCCACCGCCGACGACGCGCGACGACGACGACGACGGGCAC649870.18467004214416619No Hit
CCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGCGCGG462870.13153126380240696No Hit
CACTCCCCCCACCCGCCAGCCGCTCCTCCTCGGGCCCGCAGAGGCGCCGA438160.12450955678195308No Hit
CTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCAC419620.11924114528218722No Hit
GACGGGCCGCTCGGCGGACGGACGGACGGAATCGCGCCGGCCCGACCGCC394350.11206030609129816No Hit
GGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGG383840.10907373624973725No Hit
ACACGACCGGTCGGAGGCAGAACGGCAGCCCCTCGGCGGCCGGCCGGCGC382170.10859918138433222No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC380200.10803937714190835No Hit
GCAACACGCCCCCACCGCCGACGACGCGCGACGACGACGACGACGACGGG374000.10627755668351849No Hit
CGACGACGACGACGACGGGCACGGGACCTTCCACCCGGCCGGGGCCGACG366870.10425146315637011No Hit
AGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAG365490.10385931602208333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)