FastQCFastQC Report
Wed 8 May 2024
EGAF00006138377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24266022
Total Bases194.1 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGGA732701330.194537036189946No Hit
ATGCTCCG637096726.25468237027066No Hit
GCTGAATT552795222.78062716666127No Hit
TGAAGTAC474374319.54891081859235No Hit
CACTCGAA419890.17303619027461528RNA PCR Primer, Index 47 (100% over 8bp)
TGTAGTAC262030.10798226425410808No Hit
GCTGAATA249130.10266618896166829No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)