FastQCFastQC Report
Wed 8 May 2024
EGAF00006138408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12159938
Total Bases97.2 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGGA356272629.298882938383404No Hit
GCTGAATT301903124.827684154310656No Hit
ATGCTCCG279121322.954171312386627No Hit
TGAAGTAC267268221.979404829202252No Hit
CACTCGAA155030.12749242635941072RNA PCR Primer, Index 47 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)