FastQCFastQC Report
Sat 28 May 2022
EGAF00006138412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26552176
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC352274813.26726668277583No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC275714710.383883415054193No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC19525857.353766410707732No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC16282046.132092526051349No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC2792431.051676517962219No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT2184540.8227348297179108No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAAAG1750690.6593395584602934No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC1514630.5704353571624412No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTC1236710.46576596961394046No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT1176390.4430484341471675No Hit
GCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCT1029130.38758781954443206No Hit
GAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTA965080.36346550278967715No Hit
GTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCT920710.34675500795113745No Hit
GTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGC901160.3393921462406697No Hit
CTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACC865770.3260636717683703No Hit
ATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGC835200.314550491078396No Hit
GTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATATT778950.29336578666848245No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG772990.29112114954344986No Hit
GCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAA746590.2811784616070638No Hit
GAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCC735890.2771486600570891No Hit
GTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAA726120.2734691122866917No Hit
GCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCC725810.27335236102683264No Hit
GCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAG714270.26900620122433655No Hit
GGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGC703050.26478055885137247No Hit
GACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTG687150.2587923490715036No Hit
CTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTAC683220.2573122443900643No Hit
AAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGG672940.25344062196635037No Hit
GGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCT672790.2533841294212572No Hit
GTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTA671840.25302634330233426No Hit
GGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCC663330.2498213329107189No Hit
GGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAA655780.24697787480769937No Hit
CTTCAGAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGT650730.24507595912289826No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAC619630.2333631714402616No Hit
AGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGT615370.2317587831596175No Hit
CTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGAC612510.23068165863317566No Hit
ATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCC611520.23030880783556118No Hit
GATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAA601530.22654640433236056No Hit
CTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTG579070.21808758724708666No Hit
CCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCT564510.2126040442033828No Hit
AGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAA555390.20916929746172216No Hit
CTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCT537470.2024203214079328No Hit
CCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTG536010.2018704606356933No Hit
GGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACT523890.19730586299217057No Hit
GGATCTTCAGAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGA516770.19462435018508464No Hit
CTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTG515780.19425149938747016No Hit
CCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATC507190.19101635963847183No Hit
GCTGGATCTTCAGAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAAC505540.1903949416424477No Hit
GGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAA492380.18543866235294612No Hit
CCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCA491090.18495282646514546No Hit
GTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGACGGATCCCTCAG488560.18399998553790844No Hit
GCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACC483570.18212066687114456No Hit
CACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGACGG483490.18209053751376159No Hit
GCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAAC481340.18128081103409377No Hit
GGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGGGCTGCC481100.18119042296194482No Hit
GATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACTG475300.17900604455167818No Hit
ATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACTGG474030.17852774100322324No Hit
CCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAAT472650.17800800958836668No Hit
GCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAG469800.17693465123159774No Hit
GTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAG465470.1753038997632435No Hit
ATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTT452840.17054722746640424No Hit
ACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGA450140.1695303616547284No Hit
CACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGT449610.1693307546620661No Hit
CTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAGACTCCAGCACCTGTGT447870.1686754411389861No Hit
GGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAA443120.1668865105443712No Hit
GGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGA435370.16396772904789422No Hit
CAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCT434620.16368526632242872No Hit
CGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTA433860.16339903742729034No Hit
CCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGAC430690.16220516164098941No Hit
ACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAG424960.1600471464184329No Hit
GTGCGCTGGATCTTCAGAGTAGAGACGGATCCCTCAGGCCTGACTGCAAA424560.15989649963151797No Hit
GGTCTGGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGGG423380.1594520916101189No Hit
CTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGGGCTGCCCAGCAG420780.15847288749517177No Hit
AGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATAT420700.1584427581377888No Hit
GTATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTC420390.15832600687792972No Hit
GGCACAGAGATACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCA420340.15830717602956534No Hit
AATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGT419620.15803601181311844No Hit
CGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGACGGAT419360.15793809140162374No Hit
CCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTA418620.15765939484583108No Hit
AATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGG417500.15723758384246925No Hit
CTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCT414790.15621695186112053No Hit
GATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTC413150.1555993000347693No Hit
ATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCT412170.1552302154068277No Hit
CATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGC408080.1536898520106224No Hit
ATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAGACTCCAGCA399580.15048860778867992No Hit
CTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCC399120.15031536398372775No Hit
GCTGGCACAGAGATACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCT395910.14910642351873535No Hit
GGGGGTCTGGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACA392060.14765644819467902No Hit
GCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGA391440.14742294567496086No Hit
GAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGT390900.1472195725126257No Hit
CCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGACGGATCCCTCAGGCCT386400.1455247961598326No Hit
AAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTAT383410.14439871142764346No Hit
GGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCT383060.14426689548909288No Hit
CTACATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAGACT382280.14397313425460873No Hit
GACCCTGTCCCGGGGCTGCTGGCACAGAGATACACGGCTGAGTCCCCCCG380810.14341950731269634No Hit
GAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGA379640.14297886546097013No Hit
CAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATA378760.14264744252975725No Hit
GGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACAC377440.14215030813293797No Hit
GGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGT374190.14092630298925407No Hit
GCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGACGGATCC371300.1398378799537936No Hit
GGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGGGCTGCCC368650.13883984499048213No Hit
GACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTG368410.13874945691833318No Hit
GCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTT367830.13853101907730653No Hit
CTGGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGGGCTG366580.13806024786819732No Hit
ACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGA362420.13649352128428194No Hit
GCTTCTTTAGACCCTGTCCCGGGGCTGCTGGCACAGAGATACACGGCTGA362280.1364407949088617No Hit
CCTTGTTACTGGGGGTCTGGGAGACTCCAGCACCTGTGTGATCTGCCCCC361940.136312745139984No Hit
CCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCAC361410.1361131381473217No Hit
CTGGCACAGAGATACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTT361390.13610560580797595No Hit
GCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGT360380.13572522267101572No Hit
CCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACAT360270.13568379480461412No Hit
GCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCA360230.13566873012592265No Hit
CACAGAGATACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGA359430.13536743655209277No Hit
CCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGT357060.13447485433962172No Hit
CGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAG357010.13445602349125735No Hit
GTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTA352520.13276501330813714No Hit
AAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTT351610.13242229186790566No Hit
CGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTC351520.1323883963408498No Hit
GCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCT347290.1307953065692243No Hit
CGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGC346540.1305128438437588No Hit
CCTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAGACTCCAGCACCTGTG345400.13008350050105122No Hit
CCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGG345380.13007596816170547No Hit
CTGGATCTTCAGAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACC343370.12931896805745788No Hit
GGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAG339110.12771457977681377No Hit
CTGGGGGTCTGGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCA331700.1249238480492145No Hit
CTGTGACCTTGTTACTGGGGGTCTGGGAGACTCCAGCACCTGTGTGATCT331060.1246828131901506No Hit
AACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATG330730.12455852959094578No Hit
CCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGG328240.12362075334240027No Hit
GGGTCTGGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGG327140.12320647467838418No Hit
GATACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAG324090.12205779292815774No Hit
GTGACCTTGTTACTGGGGGTCTGGGAGACTCCAGCACCTGTGTGATCTGC323710.12191467848058855No Hit
GAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGC322020.12127819580587294No Hit
AGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCC320860.1208413201238196No Hit
AAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTT320340.12064547930083018No Hit
CCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGAT320210.12059651909508283No Hit
ACATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAGACTCC318370.11990354387527409No Hit
GCCTTGTCCAAAGAAAGCTTCTTTAGACCCTGTCCCGGGGCTGCTGGCAC317960.11974913091868629No Hit
CTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTG317600.11961354881046284No Hit
CCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCT317470.11956458860471549No Hit
CTTCTTTAGACCCTGTCCCGGGGCTGCTGGCACAGAGATACACGGCTGAG314540.11846110089056355No Hit
CTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGG313950.118238896879864No Hit
GACCTTGTTACTGGGGGTCTGGGAGACTCCAGCACCTGTGTGATCTGCCC313570.11809578243229481No Hit
CTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGACGGATCCC313290.11799032968145436No Hit
AGAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGG312880.11783591672486654No Hit
GAGATACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAG311220.11721073255916954No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAG308810.11630308566800703No Hit
GGTGCCTTGTCCAAAGAAAGCTTCTTTAGACCCTGTCCCGGGGCTGCTGG303650.11435974211680427No Hit
GCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACA300780.11327885142068958No Hit
CTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCTG298430.11239380154756433No Hit
ATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCA294970.11109070684075008No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAAAGA292280.1100776071987471No Hit
GTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCG291780.10988929871510343No Hit
AAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTG287890.10842425871235561No Hit
GTCTGGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGGGC287510.10828114426478642No Hit
GTGATCTGCCCCCAGGAGGCACAGGGCTGCCCAGCAGAGGAGCCTGGTGC285260.10743375608838988No Hit
ATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTG279720.10534729808961796No Hit
CATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAGACTCCA278810.10500457664938648No Hit
CCCGGGGCTGCTGGCACAGAGATACACGGCTGAGTCCCCCCGCTCTGTGC278730.1049744472920035No Hit
GAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTT278590.10492172091658325No Hit
CCCTGTCCCGGGGCTGCTGGCACAGAGATACACGGCTGAGTCCCCCCGCT273950.10317421818836994No Hit
CGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATT273650.10306123309818374No Hit
CCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTG269830.10162255628314606No Hit
AAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTTC268440.10109905869861666No Hit
CGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTC267380.10069984471329206No Hit
TACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGAC266150.10023660584352861No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)