FastQCFastQC Report
Sat 28 May 2022
EGAF00006138416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30261054
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC28379009.378060658429147No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC16995065.616149391227418No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC12954074.28077290368009No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC7553552.4961291830747205No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG2733480.9032996669580642No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC2445020.8079758226531039No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT1860270.6147406498134533No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTAAGATCC1137040.3757436869185059No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC1106450.3656349841614902No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT853830.2821547458327129No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTGA637070.21052472263523936No Hit
CCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCC520010.17184133771414573No Hit
GTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATT510310.16863589748063632No Hit
GAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACA490780.16218205750533343No Hit
GGAATATCTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAGATCAG484820.16021252927938331No Hit
CTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACC473550.15648827036890386No Hit
CCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATC470850.15559603442761777No Hit
ATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTC455030.15036819272719318No Hit
CCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATAT437370.1445323087556699No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAG436720.14431751121424918No Hit
ATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTC425560.14062960265693322No Hit
ATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAG418560.13831639836470996No Hit
CCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTT403060.13319430314621558No Hit
GGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATA402200.13291010947602816No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGAAAAG394460.1303523664443413No Hit
CTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCC391340.12932133824552178No Hit
GATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTT385490.12738816037273518No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG383730.12680655472211907No Hit
CCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGAC381120.12594405997887584No Hit
AATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATA379880.12553429236139627No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTGACC379770.12549794200823278No Hit
GCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGG377730.1248238081859277No Hit
GGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTG374260.12367711977249701No Hit
GCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATATCTCCTTTT359140.11868059850129477No Hit
CTTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCT353590.11684655795531775No Hit
GTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGA347930.11497616705617722No Hit
GGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACAC342420.11315534482044147No Hit
GTGCTGGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGA333760.11029358065320527No Hit
GAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGTT332190.10977476197623519No Hit
CTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGC331080.1094079538670398No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCAATGTG319730.10565725833607778No Hit
AGAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACAT309150.10216101527726035No Hit
CTCAGGAATATCTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAGA308080.10180742547830622No Hit
CTCTAGAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTA306640.10133156630962029No Hit
GCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATA304650.10067395537511682No Hit
GTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGA303140.10017496416350866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)