FastQCFastQC Report
Wed 11 May 2022
EGAF00006138418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28440857
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC19390626.817874721566934No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC14609665.136856459705134No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTC5515581.9393156823649864No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC1711360.6017258903274257No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1361160.4785931731944646No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT1346510.47344213291463055No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCA1174940.41311694651114067No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT1048970.3688250322414687No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA1038770.36523864242206205No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1036290.3643666574463632No Hit
GCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGTTTATACCAATACA769560.2705825636688796No Hit
GAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCAGA766720.2695840002289664No Hit
CCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGAC711760.2502596880255753No Hit
GTTTCATTTATAATGAGCTCCTTATTATTGTAGCTAAACATTATCTTCAG688990.24225360016401756No Hit
CCAATACATAGTATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGT639820.2249650915934073No Hit
GTCCTGTTTATACCAATACATAGTATCATGGCCCAGATTTTGTTCACATT630160.22156856947032222No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCACC614760.2161538240567083No Hit
GCTCCTTATTATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCC603980.2123635022671785No Hit
GTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTT575750.20243764103170309No Hit
GTGAGCTTTGTCTGGAGATTTAGGTGAGAAGCGATTTGGAACTGTTTCAT572140.2011683403211092No Hit
GCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGA514070.18075053082964415No Hit
GGCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTG466510.16402810927954808No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA464660.16337763661622434No Hit
GTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGT454030.15964005585345056No Hit
GTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGTTTATACCAAT451570.15877510301465247No Hit
CCTGTTTATACCAATACATAGTATCATGGCCCAGATTTTGTTCACATTTA419750.14758697320548395No Hit
AGAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCAG415930.14624383505743166No Hit
GGAGATTTAGGTGAGAAGCGATTTGGAACTGTTTCATTTATAATGAGCTC415640.1461418690723701No Hit
GAAGCGATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATTGTAGC409460.1439689387700237No Hit
ATGAGCTCCTTATTATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGA409280.1439056495379165No Hit
GTGAAGATTTAAGTGAGCTTTGTCTGGAGATTTAGGTGAGAAGCGATTTG402630.14156746401840142No Hit
CTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAG398010.1399430403943172No Hit
GTTTATACCAATACATAGTATCATGGCCCAGATTTTGTTCACATTTAATG396320.13934882482619987No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGT394590.13874054498428087No Hit
GGAACTGTTTCATTTATAATGAGCTCCTTATTATTGTAGCTAAACATTAT392030.13784043146097882No Hit
CAGAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCA384400.13515767123332467No Hit
ATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGTTTATACC381850.134261073778473No Hit
GGTGAGAAGCGATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATT379380.1333926048712245No Hit
AGCGATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATTGTAGCTA375730.132109239886829No Hit
CCTTATTATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGT372300.13090322840834226No Hit
ATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGT368270.1294862528228316No Hit
CTGGAGATTTAGGTGAGAAGCGATTTGGAACTGTTTCATTTATAATGAGC367900.12935615829016686No Hit
GCTTTGTCTGGAGATTTAGGTGAGAAGCGATTTGGAACTGTTTCATTTAT363850.1279321505677554No Hit
GAAGATTTAAGTGAGCTTTGTCTGGAGATTTAGGTGAGAAGCGATTTGGA362790.12755944731201313No Hit
GTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGAC361970.12727112969908044No Hit
CTTCAGAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGC361800.12721135653542368No Hit
GATTTAGGTGAGAAGCGATTTGGAACTGTTTCATTTATAATGAGCTCCTT356860.12547441872092674No Hit
GTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTG354770.12473956041479341No Hit
GGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGG348520.12254201763329424No Hit
GTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACACAGCAGAGGAGCC347700.12225370002036154No Hit
GTGAGAAGCGATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATTG343720.12085430477710288No Hit
ATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATTGTAGCTAAACA339870.11950061842369938No Hit
ATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCAGATTT338200.11891343499248282No Hit
GATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATTGTAGCTAAAC324160.1139768748881231No Hit
GAACTGTTTCATTTATAATGAGCTCCTTATTATTGTAGCTAAACATTATC323630.11379052326025198No Hit
CTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCAGATTTTGTT318590.11201842476125104No Hit
CTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCA315020.11076318832445872No Hit
ATTTATAATGAGCTCCTTATTATTGTAGCTAAACATTATCTTCAGAAATT313430.11020413344084534No Hit
GTATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCAT311240.10943411445020802No Hit
ATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCT309210.1087203525547771No Hit
CACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGA306890.10790462467428459No Hit
CCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACC304900.10720492705265526No Hit
GCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACACAGCAGAG303090.1065685186631331No Hit
ATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGA300740.10574224257728942No Hit
GTCACCAAGCTCCAGGGAATTGATGTGAAGATTTAAGTGAGCTTTGTCTG298650.1050073842711561No Hit
CTCCTTATTATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCCT296580.10427955810192358No Hit
ATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTC293010.10302432166513127No Hit
CACCAAGCTCCAGGGAATTGATGTGAAGATTTAAGTGAGCTTTGTCTGGA290220.10204333856747004No Hit
CAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGA290000.10196598506156127No Hit
GTCTGGAGATTTAGGTGAGAAGCGATTTGGAACTGTTTCATTTATAATGA288250.10135067308274151No Hit
AATACATAGTATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGTCG287930.10123815889232875No Hit
GATGTGAAGATTTAAGTGAGCTTTGTCTGGAGATTTAGGTGAGAAGCGAT287410.101055323332908No Hit
CCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAA285660.10044001135408823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)