FastQCFastQC Report
Sun 15 May 2022
EGAF00006138448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28365750
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC19734166.957037977137921No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC16441965.796412927562288No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC10747563.788921498638323No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC8720783.0744048720728343No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC1972040.6952187056573509No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT1444990.5094136414513982No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT1438710.5071997038682213No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC1074390.37876312101742415No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT785920.2770665327022906No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA752060.2651296017203846No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG585910.20655544098076026No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATACGGATGA506470.17854983562923596No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG475490.16762821360267222No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGAGTTCC470680.16593250663211795No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG401560.1415650917039035No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTT401230.14144875421943717No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG389260.13722887637379586No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG366040.12904294792134882No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG362930.12794655526471185No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGCCGG361230.12734724095079453No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTGTG339290.11961256092294405No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGGGGTTTG329400.11612596176727216No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG323160.11392612569736389No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA319330.11257590580189138No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTTA318750.11237143385949605No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT318430.1122586217533469No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA298100.10509152763455928No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATATGGGTTT291160.10264491508244979No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA287580.10138282964490626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)