FastQCFastQC Report
Wed 8 May 2024
EGAF00006138452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24819610
Total Bases198.5 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGGA749735730.20739246104189No Hit
ATGCTCCG650594726.2129300178367No Hit
GCTGAATT567385122.860355178828353No Hit
TGAAGTAC485792719.572938495004554No Hit
CACTCGAA426820.17196885849535912RNA PCR Primer, Index 47 (100% over 8bp)
TGTAGTAC258130.10400244000610807No Hit
GCTGAATA252260.10163737464045568No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)