FastQCFastQC Report
Fri 3 May 2024
EGAF00006138454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28911808
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC19938596.896348370880161No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC15042985.203057518920988No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTC5603271.9380558974381679No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC1769250.6119472016416269No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT1398560.483733151520652No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1388880.4803850385282027No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCA1192370.41241626950483345No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT1058970.3662759520262448No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1054100.36459151914677906No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA1048450.36263730030304575No Hit
GCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGTTTATACCAATACA778450.2692498511334884No Hit
GAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCAGA774980.26804964947193893No Hit
CCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGAC729240.2522291238237332No Hit
GTTTCATTTATAATGAGCTCCTTATTATTGTAGCTAAACATTATCTTCAG689420.23845620446843033No Hit
CCAATACATAGTATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGT648930.22445154588741045No Hit
GTCCTGTTTATACCAATACATAGTATCATGGCCCAGATTTTGTTCACATT643510.2225768793151919No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCACC626250.21660700015716763No Hit
GCTCCTTATTATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCC614730.21262246899259982No Hit
GTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTT582310.20140905750342558No Hit
GTGAGCTTTGTCTGGAGATTTAGGTGAGAAGCGATTTGGAACTGTTTCAT571590.19770122989195277No Hit
GCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGA523750.18115435741687272No Hit
GGCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTG477980.1653234553854259No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA473260.1636909044221655No Hit
GTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGT465900.16114523173369166No Hit
GTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGTTTATACCAAT456560.15791471775130772No Hit
CCTGTTTATACCAATACATAGTATCATGGCCCAGATTTTGTTCACATTTA429860.1486797366667626No Hit
GGAGATTTAGGTGAGAAGCGATTTGGAACTGTTTCATTTATAATGAGCTC419240.1450064969994267No Hit
AGAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCAG417630.14444963109882303No Hit
GAAGCGATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATTGTAGC415550.14373020186077606No Hit
CTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAG410900.14212186245841146No Hit
ATGAGCTCCTTATTATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGA407630.1409908366851357No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGT406100.14046164113984155No Hit
GTTTATACCAATACATAGTATCATGGCCCAGATTTTGTTCACATTTAATG403060.1394101676380806No Hit
GTGAAGATTTAAGTGAGCTTTGTCTGGAGATTTAGGTGAGAAGCGATTTG399770.1382722242759775No Hit
GGAACTGTTTCATTTATAATGAGCTCCTTATTATTGTAGCTAAACATTAT397300.1374179020557967No Hit
CAGAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCA390640.13511434497628097No Hit
ATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGTTTATACC385680.13339878294709206No Hit
GGTGAGAAGCGATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATT379300.13119207211115957No Hit
GTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGAC378180.13080468713682658No Hit
ATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGT375590.12990885938368157No Hit
AGCGATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATTGTAGCTA374320.1294695924931433No Hit
CTGGAGATTTAGGTGAGAAGCGATTTGGAACTGTTTCATTTATAATGAGC367250.12702422484266634No Hit
CTTCAGAAATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGC366270.126685262990125No Hit
CCTTATTATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCCTGT366140.12664029866274706No Hit
GAAGATTTAAGTGAGCTTTGTCTGGAGATTTAGGTGAGAAGCGATTTGGA365760.12650886447502696No Hit
GCTTTGTCTGGAGATTTAGGTGAGAAGCGATTTGGAACTGTTTCATTTAT364610.1261111031174529No Hit
GGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGG363360.125678753815742No Hit
GTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTG362940.12553348445036713No Hit
GTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACACAGCAGAGGAGCC361000.12486247833411179No Hit
GATTTAGGTGAGAAGCGATTTGGAACTGTTTCATTTATAATGAGCTCCTT355700.12302931729485751No Hit
GTGAGAAGCGATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATTG347500.12019310587563392No Hit
ATTTCTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCAGATTT341450.1181005352553531No Hit
ATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATTGTAGCTAAACA341290.11804519454473412No Hit
CTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCA331140.1145345182148415No Hit
GAACTGTTTCATTTATAATGAGCTCCTTATTATTGTAGCTAAACATTATC325640.11263218128731348No Hit
CTTAGAGTCCTGTTTATACCAATACATAGTATCATGGCCCAGATTTTGTT325130.11245578277221542No Hit
GATTTGGAACTGTTTCATTTATAATGAGCTCCTTATTATTGTAGCTAAAC324190.1121306560973288No Hit
CACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGA318290.1100899673932533No Hit
GTATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCAT317390.10977867589602144No Hit
GCTGTGTCCAAGGGACCTGCTTGGAGGAGGCAGAAGACCACACAGCAGAG316220.10937399694962004No Hit
ATTTATAATGAGCTCCTTATTATTGTAGCTAAACATTATCTTCAGAAATT313230.10833981741992751No Hit
CCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCTGTGTGACC313070.10828447670930853No Hit
ATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGA311590.10777257513608281No Hit
ATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGTCGTTTCCCATCT311280.1076653525092585No Hit
GTCACCAAGCTCCAGGGAATTGATGTGAAGATTTAAGTGAGCTTTGTCTG303090.1048325998844486No Hit
CAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGA301160.10416505256260694No Hit
CTCCTTATTATTGTAGCTAAACATTATCTTCAGAAATTTCTTAGAGTCCT299460.10357705751228011No Hit
CCCATCTGTGTGACCAGGTATTTTGGAGTCTGGGAAACAGCTGTGTCCAA296170.10243911415017698No Hit
ATTTAATGGACTTGTCGTTTCCCATCTGTGTGACCAGGTATTTTGGAGTC294220.10176464923950794No Hit
ATTTTGGAGTCTGGGAAACAGCTGTGTCCAAGGGACCTGCTTGGAGGAGG293230.10142222859255291No Hit
AATACATAGTATCATGGCCCAGATTTTGTTCACATTTAATGGACTTGTCG291750.1009103270193272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)