FastQCFastQC Report
Wed 11 May 2022
EGAF00006138458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26097553
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC344797013.211851701192062No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC270017310.346460451675297No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC19320667.403245813889141No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC16114296.17463637299635No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC2734101.0476461145610088No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT2135560.8182989416670597No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAAAG1714230.6568546867210118No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC1491980.5716934457418287No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTC1232170.4721400508315856No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT1155870.4429035932985748No Hit
GCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCT1017320.3898143247376488No Hit
GAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTA951310.3645207656058788No Hit
GTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCT904770.3466876760438038No Hit
GTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGC878550.33664075708553975No Hit
CTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACC848360.3250726227091099No Hit
ATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGC817200.31313280597610055No Hit
GTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATATT765120.29317691202696283No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG759000.2908318645813268No Hit
GCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAA735090.28167008608048427No Hit
GAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCC734450.28142485236067916No Hit
GCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCC713470.2733857844833192No Hit
GTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAA711350.2725734477864649No Hit
GCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAG703390.26952335339638933No Hit
GGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGC689930.2643657817267389No Hit
GACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTG680180.26062979927658353No Hit
CTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTAC671070.2571390505462332No Hit
GGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCT663030.254058301941182No Hit
GTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTA662200.25374026446080983No Hit
AAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGG657480.2519316657772474No Hit
GGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAA647910.24826465531078717No Hit
GGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCC645280.24725689799346323No Hit
CTTCAGAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGT644700.24703465493488988No Hit
AGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGT610190.2338111929497758No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAC606300.2323206317465856No Hit
CTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGAC605590.23204857558867684No Hit
ATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCC603010.23105997715571264No Hit
GATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAA593730.2275040882185391No Hit
CTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTG570310.2185300667844223No Hit
CCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCT561100.21500100028535243No Hit
AGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAA548860.21031090539408043No Hit
CTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCT530990.2034635201238982No Hit
CCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTG528520.20251707123652551No Hit
GGATCTTCAGAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGA512990.19656632175438057No Hit
GGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACT508420.1948151997238975No Hit
CTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTG506850.19421361075500068No Hit
GCTGGATCTTCAGAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAAC502800.1926617411218592No Hit
CCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATC494290.18940089900382615No Hit
GGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAA483950.18543884171822547No Hit
GTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGACGGATCCCTCAG481460.18448472927710885No Hit
CCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCA479020.183549775720352No Hit
CACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGACGG476660.18264547637857081No Hit
GCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAAC473540.1814499619945211No Hit
GCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACC473220.18132734513461857No Hit
GGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGGGCTGCC468300.1794421109136171No Hit
GATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACTG465470.178357718058854No Hit
CCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAAT464550.17800519458663425No Hit
GCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAG462350.1771622036748043No Hit
ATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACTGG461920.17699743726931028No Hit
GTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAG461150.17670239045016978No Hit
ATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTT445040.1705293979094515No Hit
ACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGA438050.16785098587595548No Hit
CACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGT437380.16759425682553458No Hit
CTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAGACTCCAGCACCTGTGT436630.167306873560138No Hit
GGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAA431280.16525687293364247No Hit
CGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTA430870.16509977008189236No Hit
CCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGAC429180.16445219979053208No Hit
GGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGA427460.16379313416855595No Hit
GTGCGCTGGATCTTCAGAGTAGAGACGGATCCCTCAGGCCTGACTGCAAA423920.16243668515588416No Hit
CAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCT422930.16205733924556068No Hit
GGCACAGAGATACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCA417550.15999584328844932No Hit
ACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAG415720.15929462812088166No Hit
CGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGACGGAT414690.1588999551030704No Hit
GATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTC414290.15874668402819223No Hit
GTATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTC412090.1579036931163623No Hit
CCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTA411760.1577772444795878No Hit
AGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATAT409550.1569304217908859No Hit
AATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGG406360.1557080849687325No Hit
AATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGT405780.15548584191015916No Hit
GGTCTGGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGGG405440.1553555614965127No Hit
CTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCT405060.15520995397537846No Hit
CATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGC403450.1545930378989938No Hit
ATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCT401690.15391864516952986No Hit
CTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGGGCTGCCCAGCAG400490.15345883194489537No Hit
CTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCC395540.15156210239327803No Hit
GCTGGCACAGAGATACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCT392210.15028612069491726No Hit
ATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAGACTCCAGCA387100.14832808271334866No Hit
GAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGT383950.14712107299868307No Hit
CCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGACGGATCCCTCAGGCCT382940.14673406353461566No Hit
GCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGA381250.14608649324325543No Hit
GGGGGTCTGGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACA380110.14564967067985263No Hit
GGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCT376740.14435836187400405No Hit
GAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGA375520.14389088509562561No Hit
GACCCTGTCCCGGGGCTGCTGGCACAGAGATACACGGCTGAGTCCCCCCG375120.14373761402074747No Hit
GGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACAC374750.14359583827648514No Hit
AAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTAT374360.14344639897847894No Hit
CAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATA372770.1428371464558382No Hit
GGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGT372580.1427643426952711No Hit
CTACATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAGACT372100.1425804174054173No Hit
GCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGACGGATCC367410.14078331405247074No Hit
CCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCAC359970.1379324720597368No Hit
GCTTCTTTAGACCCTGTCCCGGGGCTGCTGGCACAGAGATACACGGCTGA358050.13719677090032156No Hit
ACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGA357060.1368174249899981No Hit
GCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTT356300.1365262099477296No Hit
GACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTG356280.13651854639398567No Hit
GCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCA354280.13575219101959482No Hit
CTGGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGGGCTG353800.13556826572974104No Hit
CACAGAGATACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGA352920.13523106936500906No Hit
GGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGGGCTGCCC351830.13481340568596603No Hit
CGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAG351190.13456817196616097No Hit
GCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGT349890.13407004097280692No Hit
CTGGCACAGAGATACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTT349010.13373284460807494No Hit
CCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGT347320.13308527431671469No Hit
GTACCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTA346900.1329243396880926No Hit
CCTTGTTACTGGGGGTCTGGGAGACTCCAGCACCTGTGTGATCTGCCCCC346140.13263312464582408No Hit
CCAGTAAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACAT343900.1317748066265063No Hit
CGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGC343370.13157172245229276No Hit
CGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTC341750.13095097459903615No Hit
AAAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTT341720.13093947926842028No Hit
GCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCT341420.13082452596226168No Hit
CCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGG340430.1304451800519382No Hit
CTGGATCTTCAGAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACC339260.12999686215791956No Hit
CCTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAGACTCCAGCACCTGTG335140.12841817008667442No Hit
CCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGG328280.1257895711525138No Hit
GGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAG327380.12544471123403791No Hit
AACTCTGGGCCCTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATG323650.12401545846079899No Hit
GATACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAG321550.1232107853176886No Hit
GAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGC320550.12282760763049318No Hit
AAAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTT319990.12261302812566373No Hit
CTGGGGGTCTGGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCA319890.1225747103569442No Hit
CCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTGGAT319140.12228732709154762No Hit
CTGTGACCTTGTTACTGGGGGTCTGGGAGACTCCAGCACCTGTGTGATCT318670.12210723357856576No Hit
AGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCC317180.1215362988246446No Hit
GGGTCTGGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGG313770.1202296629113082No Hit
GTGACCTTGTTACTGGGGGTCTGGGAGACTCCAGCACCTGTGTGATCTGC313040.11994994319965555No Hit
CTGCCCCAGGCTTTGTCGGTACCAGTAAAGGGCAGTATGACCTGAAATTG311690.11943265332194171No Hit
CCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCT310510.11898050365105112No Hit
AGAGTAGAGACGGATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGG309920.1187544288156058No Hit
CTTCTTTAGACCCTGTCCCGGGGCTGCTGGCACAGAGATACACGGCTGAG309230.11849003621144097No Hit
GAGATACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAG308820.11833293335969085No Hit
CTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGACGGATCCC307460.11781181170510506No Hit
ACATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAGACTCC306830.11757040976217197No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAG306540.11745928823288529No Hit
GCCTTGTCCAAAGAAAGCTTCTTTAGACCCTGTCCCGGGGCTGCTGGCAC305460.11704545633071423No Hit
CTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTGGG305100.11690751236332388No Hit
GACCTTGTTACTGGGGGTCTGGGAGACTCCAGCACCTGTGTGATCTGCCC302340.1158499419466645No Hit
GGTGCCTTGTCCAAAGAAAGCTTCTTTAGACCCTGTCCCGGGGCTGCTGG295730.11331713743430276No Hit
GCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACA292920.1122404081332836No Hit
CTGAGCTCTACATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCTG291210.11158517428817942No Hit
ATCCCTCAGGCCTGACTGCAAAGAACCGATCGTTGGGCAGCCCTGAGTCA288350.11048928610280051No Hit
GTGCCTTGGAAGTAAATTAGAAACTCTGGGCCCTGCCCCAGGCTTTGTCG288140.11040881878848947No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAAAGA285760.10949685589296436No Hit
AAGAACCGATCGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTG285350.10933975304121424No Hit
GAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTGTGACCTT274270.10509414426708895No Hit
GTCTGGGAGACTCCAGCACCTGTGTGATCTGCCCCCAGGAGGCACAGGGC273350.10474162079486915No Hit
CCCGGGGCTGCTGGCACAGAGATACACGGCTGAGTCCCCCCGCTCTGTGC273290.10471863013363743No Hit
GTGATCTGCCCCCAGGAGGCACAGGGCTGCCCAGCAGAGGAGCCTGGTGC272860.10455386372814339No Hit
CCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATTAGAAACTCTG272860.10455386372814339No Hit
ATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTTCCCTTCTCTG272770.10451937773629581No Hit
CGTTGGGCAGCCCTGAGTCATCTGCCGCACCCGTGCCTTGGAAGTAAATT272350.10435844310767374No Hit
CATATTTTCCCTTCTCTGTGACCTTGTTACTGGGGGTCTGGGAGACTCCA270800.10376451769252082No Hit
CCCTGTCCCGGGGCTGCTGGCACAGAGATACACGGCTGAGTCCCCCCGCT269610.10330853624475828No Hit
AAGGGCAGTATGACCTGAAATTGGATCACACCTGAGCTCTACATATTTTC265860.10187161991777544No Hit
TACACGGCTGAGTCCCCCCGCTCTGTGCGCTGGATCTTCAGAGTAGAGAC263740.10105928322092113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)