FastQCFastQC Report
Thu 12 May 2022
EGAF00006138494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90316539
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATGAGGATCTCGTA1435790.15897309793945935TruSeq Adapter, Index 3 (97% over 36bp)
AGGTTACCACGATCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1386660.15353334121893222Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGC1248750.13826371269607662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)