FastQCFastQC Report
Sat 28 May 2022
EGAF00006138703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13964582
Sequences flagged as poor quality0
Sequence length35-51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT596240.42696587695929605No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA403540.28897391987816035No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA392510.28107536623724216No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCAAGGCAATCTCGTATGCCG378470.2710213596081859No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA336560.24100972016204997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330800.2368849994937192No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA310210.22214055529911314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215490.15431181541989586No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA211960.15178399181586674No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA185090.13254245633703893No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCAAGGCAATCGCGTATGCCG181410.12990721813227205No Hit
ATACACATCTCCGAGCCCACGAGACGATCAAGGCAATCTCGTATGCCGT167830.12018261627881163No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA161600.11572132986150249No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA160740.11510548615060587No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA154300.11049381929226382No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA153530.10994242434180987No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA146220.1047077527991887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)