FastQCFastQC Report
Wed 11 May 2022
EGAF00006138705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12468289
Sequences flagged as poor quality0
Sequence length35-51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGTGCTCTATCTCGTATGCCG1544581.2388067039511195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1137580.9123785950101092No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1026100.8229677704775692No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1001680.8033820839411084No Hit
ATACACATCTCCGAGCCCACGAGACCTAGTGCTCTATCTCGTATGCCGT969820.7778292594918196No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA855840.6864133482950227No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGTGCTCTATCGCGTATGCCG840590.6741823196430561No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA784150.6289154831107941No Hit
ATACACATCTCCGAGCCCACGAGACCTAGTGCTCTATCGCGTATGCCGT730490.5858783029491857No Hit
ACACATCTCCGAGCCCACGAGACCTAGTGCTCTATCTCGTATGCCGTCTT680560.5458327120906485RNA PCR Primer, Index 32 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT622200.4990259690002373No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA535710.42965799076360833No Hit
ACACATCTCCGAGCCCACGAGACCTAGTGCTCTATCGCGTATGCCGTCTT510130.40914194401493253No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA464740.37273759053868577No Hit
TCTCCGAGCCCACGAGACCTAGTGCTCTATCTCGTATGCCGTCTTCTGCTT438920.3520290554702413TruSeq Adapter, Index 2 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416000.3336464209323348No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA404450.32438292054346834No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA401260.32182442995987665No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400350.3210945784140871No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA398020.3192258376429998No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA357710.2868958202685228No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA323720.2596346619812871No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT313120.2511330945248382No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA311870.25013055119270977No Hit
TCTCCGAGCCCACGAGACCTAGTGCTCTATCGCGTATGCCGTCTTCTGCTT304270.24403508773336904Illumina PCR Primer Index 2 (95% over 24bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA281800.2260133687950287No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA276270.22157811709369266No Hit
CATCTCCGAGCCCACGAGACCTAGTGCTCTATCTCGTATGCCGTCTTCTG275490.2209525300544445RNA PCR Primer, Index 32 (95% over 24bp)
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA256150.20544117961975375No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG233470.1872510334016159No Hit
CATCTCCGAGCCCACGAGACCTAGTGCTCTATCGCGTATGCCGTCTTCTG230940.185221885697388RNA PCR Primer, Index 27 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT229190.18381832503240822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT219060.17569371386883958No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA217390.17435431597711604No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA213210.17100181107447862No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA200450.16076784874011182No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA199240.1597973867946115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT191470.1535655774421013No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA176310.14140673191004796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT174730.14013951713823766No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA173760.13936154351250601No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA172140.13806224735406758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT169100.13562406197033128No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA168480.1351268004775956No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT166320.13339440559967772No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT163680.1312770340822225No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA162300.13017022624355273No Hit
CACATCTCCGAGCCCACGAGACCTAGTGCTCTATCTCGTATGCCGTCTT161310.12937621192450705No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA155820.12497304160979907No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG153550.12315242291865387No Hit
CACATCTCCGAGCCCACGAGACCTAGTGCTCTATCGCGTATGCCGTCTT139180.11162718477250566No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA135740.10886818552248828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT133020.10668665123177688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG130730.10484999184731762No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT127130.1019626670507878No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN124810.10010194662635748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)