FastQCFastQC Report
Wed 11 May 2022
EGAF00006138706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12468289
Sequences flagged as poor quality0
Sequence length35-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7916346.349179105489133No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1313451.0534324316672479No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1220880.9791880826631464No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1038960.8332819362785062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980250.786194481055099No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA685800.5500353737389307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT511830.41050540294662724No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA510160.4091660050549037No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA507390.4069443690309071No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA480170.3851129854304789No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA430290.3451074963052268No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA417470.3348254118909178No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410960.3296041662171931No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA357300.2865669860555847No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA307580.24668982247684507No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA283210.2271442376736696No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA281380.22567651423543358No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA279970.22454564535679275No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA251310.20155933183775257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT243320.19515107485878777No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA229790.18429954583182986No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA223910.1795835819974978No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT215220.1726139007525411No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG200500.16080795047339697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT196100.15727899794430494No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA193000.1547926904806265No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA187180.15012484872623663No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA182710.14653975377054543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT178130.14286643500162693No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA174730.14013951713823766No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA173940.1395059097523325No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA169330.13580852994344292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT164720.13211115013455335No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT156840.12579111696881584No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA150930.12105109209451274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG147130.11800336036484237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT146160.11722538673911072No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA145770.11691259321948665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT145450.11665594212646177No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT142820.1145465909556636No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA131400.10538735507333846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT131390.10537933472668141No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA127300.10209901294395726No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126310.10130499862491156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)