FastQCFastQC Report
Tue 10 May 2022
EGAF00006138711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5256131
Sequences flagged as poor quality0
Sequence length35-51
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA844101.6059340986744812No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA707341.3457427145556304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT671381.277327372548363No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA559691.0648326687443672No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA504660.9601358870241248No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA461740.8784788659186767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374200.7119305055372479No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA349490.6649187396585055No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA325110.6185348120128665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285450.5430800716344398No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA279110.5310179673984534No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA268210.510280280305038No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA262830.5000446145653523No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA228020.4338171936734454No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA202020.38435115106529877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT196050.3729929866664282No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA194360.3697776938968987No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA189030.35963715516222866No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA185430.35278801080110067No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA180070.3425903958634212No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA176040.3349231592591585No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA170290.3239835536823569No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT152210.28958562866869186No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT148290.2821276714754636No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT139140.26471942955759664No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT137360.2613329081790389No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA136410.2595254950837413No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA135340.257489777176406No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA132360.25182020767747226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG132190.251496775860419No Hit
GGTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAA128960.24535157133640695No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA128100.2437153868501375No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA127850.24323975182505916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT124830.23749408072211292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT119420.2272013387794178No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA119330.2270301101703896No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA117310.22318697916775665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT107930.20534115302681763No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA104050.1979592974376019No Hit
ACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA101860.19379273461791574No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT100000.19025401003133294No Hit
GTATCAACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA94560.17990419188562842No Hit
GAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA94400.17959978546957828No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA93570.17802067718631823No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA92120.1752619940408639No Hit
GAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA89390.17006805956700852No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83560.1589762507821818No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA82170.15633172004274626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT81770.15557070400262094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTT78970.15024359172174362No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT78780.14988210910268407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG77220.14691414654619528No Hit
CATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76040.14466914922782556No Hit
ACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75620.14387008238569396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT75530.14369885377666577No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA75160.14299491393954983No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATT73430.13970351956600777No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA72840.1385810209068229No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG72390.1377248778616819No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATT71360.1357652615583592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT69820.13283534980387665No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA69520.13226458777378267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT68270.129886412648391No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT65900.1253773926106484No Hit
GAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA65420.12446417336249802No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT64030.12181964262306247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT63670.12113472818694968No Hit
TATCAACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA63580.12096349957792148No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63570.12094447417691835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTT61940.11784333381340763No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCNNNNN61820.11761502900137003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT59610.11341041537967755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGT59060.11236401832450524No Hit
ATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA58940.11213571351246764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT57980.11030927501616684No Hit
GCCCATGTACTCTGCGTTGATACCACTGGCAAAAAAAAAAAAAAAAAAAAA57810.10998584319911357Clontech SMART CDS Primer II A (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT56340.10718910925165298No Hit
GAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56010.10656127101854958No Hit
ATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA55710.10599050898845558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG55560.10570512797340857No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTT54450.10359330846206077No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTT54220.10315572423898872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT53760.10228055579284458No Hit
ATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA53360.10151953975271925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTT53090.10100585392563464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)