FastQCFastQC Report
Wed 11 May 2022
EGAF00006138715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9217875
Sequences flagged as poor quality0
Sequence length35-51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCCATTAATCTCGTATGCCG1611051.747745548730049No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1167081.2661052574481646No Hit
ATACACATCTCCGAGCCCACGAGACCTTGCCATTAATCTCGTATGCCGT917650.9955114383738117No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCCATTAATCGCGTATGCCG859250.9321562724597589No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA725820.7874049062283877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT688390.746799018211898No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA660470.7165100416310701No Hit
ATACACATCTCCGAGCCCACGAGACCTTGCCATTAATCGCGTATGCCGT604950.6562792400634636No Hit
ACACATCTCCGAGCCCACGAGACCTTGCCATTAATCTCGTATGCCGTCTT549040.5956253474906092RNA PCR Primer, Index 45 (95% over 22bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA506890.5498989734618879No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA449900.4880734442591161No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436690.4737425925172558No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA413620.44871513228374216No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA406050.4405028273870062No Hit
TCTCCGAGCCCACGAGACCTTGCCATTAATCTCGTATGCCGTCTTCTGCTT391100.4242843388525012Illumina PCR Primer Index 8 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCTTGCCATTAATCGCGTATGCCGTCTT365330.396327787044194No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA364810.3957636657038634No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA324590.35213104972675374No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA282840.30683861519059435No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA276730.30021018944170974No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA249290.2704419402519561No Hit
TCTCCGAGCCCACGAGACCTTGCCATTAATCGCGTATGCCGTCTTCTGCTT249220.2703660008407578TruSeq Adapter, Index 12 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT242250.26280460518286486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG214200.23237459826695417No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA212780.23083411306835905No Hit
CATCTCCGAGCCCACGAGACCTTGCCATTAATCTCGTATGCCGTCTTCTG210350.22819793065104485RNA PCR Primer, Index 45 (96% over 25bp)
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA194760.21128513892844067No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA192900.20926732028802736No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA183750.19934095439567145No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA175330.19020652807724123No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT168500.182797011241745No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA164760.17873967698629023No Hit
CATCTCCGAGCCCACGAGACCTTGCCATTAATCGCGTATGCCGTCTTCTG159850.1734130697150916TruSeq Adapter, Index 12 (95% over 22bp)
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA156470.16974628100294267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT156330.16959440218054594No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA154200.16728367438265326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT150110.16284664307120678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT148860.1614905821569505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG144510.15677149017533867No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA141600.15361458036695008No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA140470.15238870130046242No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT137620.14929688241595812No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT137060.14868936712637132No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA134750.14618336655682573No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN134330.14572773008963563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG131880.14306985069769332No Hit
CACATCTCCGAGCCCACGAGACCTTGCCATTAATCTCGTATGCCGTCTT131420.14257082028124704No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA130110.14114966844310647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT124210.13474906092781688No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA124150.1346839700039326No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA120210.1304096660021968No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA118750.12882578685434548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT117870.12787111997070907No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCCATTAATCTCGTATGCCGT109260.11853057239331191No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT105300.11423457141694804No Hit
CACATCTCCGAGCCCACGAGACCTTGCCATTAATCGCGTATGCCGTCTT105290.114223722929634No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA105240.11416948049306375No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA101130.10971075220698914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT100960.10952632792265028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG96860.10507844812388972No Hit
GTACATGGGAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCG93860.10182390192967467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)