FastQCFastQC Report
Tue 10 May 2022
EGAF00006138719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6031257
Sequences flagged as poor quality0
Sequence length35-51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTCTCATATATCTCGTATGCCG623101.0331179719252555No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT598890.9929770858711542No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA492470.816529622266138No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA441850.7326001859977116No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA409470.6789132016758695No Hit
ATACACATCTCCGAGCCCACGAGACCGTCTCATATATCTCGTATGCCGT381470.6324883850912008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347280.5758003679829926No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA343240.5691019301614904No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTCTCATATATCGCGTATGCCG316290.5244180441987466No Hit
ATACACATCTCCGAGCCCACGAGACCGTCTCATATATCGCGTATGCCGT267400.4433569983835874No Hit
ACACATCTCCGAGCCCACGAGACCGTCTCATATATCTCGTATGCCGTCTT258150.4280202286190093TruSeq Adapter, Index 2 (95% over 21bp)
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA228700.3791912697469201No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222950.3696576020554256No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA222240.3684804013491715No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA197910.32814055179542173No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA181820.30146286255087457No Hit
ACACATCTCCGAGCCCACGAGACCGTCTCATATATCGCGTATGCCGTCTT180850.29985457426204853No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA174670.2896079540301466No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA163500.27108776827119124No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA160710.26646186690436174No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA143630.23814272878771373No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA139140.23069817784252936No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA137300.2276474041812511No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT133860.2219437838579918No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA133310.2210318678179358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG117060.19408889390719047No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA109950.18230030655301208No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA109840.1821179233450009No Hit
CATCTCCGAGCCCACGAGACCGTCTCATATATCTCGTATGCCGTCTTCTG105390.174739693566366TruSeq Adapter, Index 2 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT99640.16520602587487151No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT97110.16101121209061395No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA96130.15938634351015055No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA90140.14945474881935888No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA89510.14841019044620382No Hit
CATCTCCGAGCCCACGAGACCGTCTCATATATCGCGTATGCCGTCTTCTG88760.14716666857340022Illumina PCR Primer Index 6 (95% over 22bp)
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA87050.14433143870340792No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT82960.1375500994237188No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA80390.1332889644729117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT79960.1325760119325043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG79440.13171383676736043No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77450.12841435873152146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT77260.12809933319041122No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA73280.12150037711873328No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA73260.12146721653545853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT73250.12145063624382116No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT70260.11649312904424403No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA68890.11422162908992271No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG66220.1097946912227418No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA65530.10865065109976245No Hit
CACATCTCCGAGCCCACGAGACCGTCTCATATATCTCGTATGCCGTCTT64830.10749003068514573TruSeq Adapter, Index 2 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT61550.10205169502808453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)