FastQCFastQC Report
Wed 11 May 2022
EGAF00006138729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10457052
Sequences flagged as poor quality0
Sequence length35-51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAACTGAGCGATCTCGTATGCCG1420191.3581169912897058No Hit
ATACACATCTCCGAGCCCACGAGACGAACTGAGCGATCTCGTATGCCGT996460.9529071864613468No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACTGAGCGATCGCGTATGCCG786010.7516554378805805No Hit
ATACACATCTCCGAGCCCACGAGACGAACTGAGCGATCGCGTATGCCGT729270.6973954035994083No Hit
ACACATCTCCGAGCCCACGAGACGAACTGAGCGATCTCGTATGCCGTCTT558680.5342614725450348Illumina PCR Primer Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGAACTGAGCGATCGCGTATGCCGTCTT439450.4202427223274781No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434560.4155664521893933No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA422300.4038423066080192No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA415700.3975307763602973No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA384490.3676848886282673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236150.22582846484841043No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA211970.20270531312266593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206400.19737876411057342No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA178150.17036350206540046No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA174480.16685390873068243No Hit
CATCTCCGAGCCCACGAGACGAACTGAGCGATCTCGTATGCCGTCTTCTG169930.16250277802960147Illumina Paired End PCR Primer 2 (96% over 25bp)
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA168200.16084839207072893No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA150260.14369250530646688No Hit
CATCTCCGAGCCCACGAGACGAACTGAGCGATCGCGTATGCCGTCTTCTG147480.1410340122627295RNA PCR Primer, Index 33 (95% over 23bp)
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA136610.130639113203224No Hit
CACATCTCCGAGCCCACGAGACGAACTGAGCGATCTCGTATGCCGTCTT128650.12302702520748678Illumina Paired End PCR Primer 2 (95% over 22bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA114400.1093998576271783No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA111130.10627278127717066No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA109990.10518260787074599No Hit
CACATCTCCGAGCCCACGAGACGAACTGAGCGATCGCGTATGCCGTCTT109990.10518260787074599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)