FastQCFastQC Report
Sat 28 May 2022
EGAF00006138733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16183051
Sequences flagged as poor quality0
Sequence length35-51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2258491.3955897438622669No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTACATACATCTCGTATGCCG1989931.2296383419912598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830881.131356503789057No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1510000.9330749807313836No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1424530.8802604651001842No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1114500.6886834874338591No Hit
ATACACATCTCCGAGCCCACGAGACTTCTACATACATCTCGTATGCCGT1112730.6875897505359156No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA1078880.6666728047758115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1008740.6233311629556132No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTACATACATCGCGTATGCCG1002590.619530890683098No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA891600.5509467899470872No Hit
ATACACATCTCCGAGCCCACGAGACTTCTACATACATCGCGTATGCCGT789070.48759038082497547No Hit
ACACATCTCCGAGCCCACGAGACTTCTACATACATCTCGTATGCCGTCTT773930.47823491379962896TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA728450.4501314368965407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690490.42667479698358485No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA655100.40480623832922485No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA625590.3865711107256598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT574820.3551987817377576No Hit
ACACATCTCCGAGCCCACGAGACTTCTACATACATCGCGTATGCCGTCTT565600.34950146298123885RNA PCR Primer, Index 39 (95% over 21bp)
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA556970.3441687231907012No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA496140.3065800138675952No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA470810.29092783554843893No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA470670.29084132528532475No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA435590.2691643250707175No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA430490.2660128797715585No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA419980.25951843073348774No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT417900.25823313539579157No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA405150.2503545221478941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT392630.24261803290368425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT384800.237779637473799No Hit
TCTCCGAGCCCACGAGACTTCTACATACATCTCGTATGCCGTCTTCTGCTT380760.2352831984525044RNA PCR Primer, Index 39 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT380180.23492479879103143No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA350900.216831795191154No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA348310.21523135532354193No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT334980.20699434241417147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT334830.20690165284654913No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA328300.202866567002724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG304930.1884255323671661No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA299730.18521229402292558No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA292660.1808435257356601No Hit
CATCTCCGAGCCCACGAGACTTCTACATACATCTCGTATGCCGTCTTCTG291620.18020087806681198TruSeq Adapter, Index 11 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT287500.17765500460945222No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA286950.17731514286150368No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA281880.17418223547586917No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA278980.17239023716850427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT277500.17147570010129734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTT264240.163281942323484No Hit
ACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA250360.15470506766616504No Hit
CATCTCCGAGCCCACGAGACTTCTACATACATCGCGTATGCCGTCTTCTG249080.15391411668912125RNA PCR Primer, Index 39 (95% over 24bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN231610.14311887171337467No Hit
GGTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAA227610.14064714991011273No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA227100.14033200538019686No Hit
GAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA223290.13797769036258983No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTT219300.13551214786383606No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA215770.1333308533724574No Hit
GAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA213540.13195286846713886No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA208050.12856043029216185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT207370.1281402375856073No Hit
GAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA206550.1276335346159386No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA203220.12557582621472305No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA202280.12499497159095649No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT201410.12445737209874702No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATT201000.12420402061391267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT195460.12078068591639488No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA193010.11926675631189693No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA184760.11416883009266919No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGT184600.11406996122053871No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTT182510.11277848657833434No Hit
CACATCTCCGAGCCCACGAGACTTCTACATACATCTCGTATGCCGTCTT182230.112605466052106RNA PCR Primer, Index 39 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG181670.11225942499964933No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATT180290.11140668097752396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG168160.10391118460913211No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT167760.10366401242880591No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTT167200.10331797137634925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT165860.10248994457225649No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162770.10058053947923663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)