FastQCFastQC Report
Wed 11 May 2022
EGAF00006138735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29949356
Sequences flagged as poor quality0
Sequence length35-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTGAGTAGATCTCGTATGCCG3549311.1851039468094071No Hit
ATACACATCTCCGAGCCCACGAGACGACTGAGTAGATCTCGTATGCCGT2537040.8471100346865555No Hit
ACACATCTCCGAGCCCACGAGACGACTGAGTAGATCTCGTATGCCGTCTT1894530.6325778757980639No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTGAGTAGATCGCGTATGCCG1776860.5932882162808443No Hit
ATACACATCTCCGAGCCCACGAGACGACTGAGTAGATCGCGTATGCCGT1739750.5808972987599467No Hit
ACACATCTCCGAGCCCACGAGACGACTGAGTAGATCGCGTATGCCGTCTT1366350.4562201604602116No Hit
CATCTCCGAGCCCACGAGACGACTGAGTAGATCTCGTATGCCGTCTTCTG828100.27650010237281897Illumina PCR Primer Index 11 (95% over 23bp)
CATCTCCGAGCCCACGAGACGACTGAGTAGATCGCGTATGCCGTCTTCTG801200.26751827318089916Illumina PCR Primer Index 9 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800170.2671743592750375No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA545360.1820940657288257No Hit
TCTCCGAGCCCACGAGACGACTGAGTAGATCGCGTATGCCGTCTTCTGCTT507760.16953953867989682Illumina PCR Primer Index 9 (96% over 25bp)
CACATCTCCGAGCCCACGAGACGACTGAGTAGATCTCGTATGCCGTCTT435640.1454588873296641RNA PCR Primer, Index 23 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432900.14454400956067304No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA418730.13981268912760594No Hit
CACATCTCCGAGCCCACGAGACGACTGAGTAGATCGCGTATGCCGTCTT384380.12834332731561907No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA344750.11511098936484644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337870.11281377803248925No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA334390.11165181648647136No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA331600.11072024386768115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)