FastQCFastQC Report
Wed 11 May 2022
EGAF00006138749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7388184
Sequences flagged as poor quality0
Sequence length35-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT625320.8463784875958693No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCCATAAGATCTCGTATGCCG571830.7739790996001182No Hit
ATACACATCTCCGAGCCCACGAGACATTCCATAAGATCTCGTATGCCGT467390.6326182455661635RNA PCR Primer, Index 28 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355590.4812955389308117No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA327010.4426121493454955No Hit
ACACATCTCCGAGCCCACGAGACATTCCATAAGATCTCGTATGCCGTCTT295290.3996787302536049RNA PCR Primer, Index 28 (95% over 24bp)
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA294630.3987854119496754No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA281200.38060773797728914No Hit
ATACACATCTCCGAGCCCACGAGACATTCCATAAGATCGCGTATGCCGT280630.3798362358057136No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCCATAAGATCGCGTATGCCG257370.34835353315510276No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA254970.3451051029589951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233500.31604518782964797No Hit
TCTCCGAGCCCACGAGACATTCCATAAGATCTCGTATGCCGTCTTCTGCTT194240.2629062838716523Illumina PCR Primer Index 9 (96% over 28bp)
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA177570.24034322913452075No Hit
ACACATCTCCGAGCCCACGAGACATTCCATAAGATCGCGTATGCCGTCTT175860.23802872261979396No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA168270.2277555621246033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT154670.20934779101332615No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA153350.20756115440546688No Hit
CATCTCCGAGCCCACGAGACATTCCATAAGATCTCGTATGCCGTCTTCTG132040.17871780128919365RNA PCR Primer, Index 28 (96% over 27bp)
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA126600.17135469284468283No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA125800.17027188277931357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG125020.16921614296557855No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA119630.16192071015015327No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA118570.16048598681353904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT116230.157318767372334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT113860.1541109425536776No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA111770.15128210125790045No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108420.14674783410916675No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA106130.1436482902970473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT96440.13053275338026232No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA93660.1267699884031042No Hit
CATCTCCGAGCCCACGAGACATTCCATAAGATCGCGTATGCCGTCTTCTG93600.12668877764820152TruSeq Adapter, Index 9 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT92370.1250239571726963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT89080.12057090077886529No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA88390.1196369770974843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG87160.1179721566219791No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT85380.11556290422653254No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA79730.10791555813986224No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA76390.10339482611694566No Hit
GTACTGGTTCACTATCGGTCAATCAGGAGTATTTAGCCTTGGAGGATGGT75620.10235262142902776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG74240.10048477406626581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)