FastQCFastQC Report
Tue 10 May 2022
EGAF00006138753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5516037
Sequences flagged as poor quality0
Sequence length35-51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA533630.9674155557694772No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT518050.9391706400809132No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA497220.9014080217373451No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA460640.8350922954287653No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA459660.8333156575998311No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATCGCGCATCTCGTATGCCG390680.7082621091917983No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA312600.5667112095150921No Hit
ATACACATCTCCGAGCCCACGAGACAACATCGCGCATCTCGTATGCCGT302930.5491805076724466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290820.5272263402149042No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA259710.47082715362496663No Hit
ACACATCTCCGAGCCCACGAGACAACATCGCGCATCTCGTATGCCGTCTT228720.4146455145242862No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA224060.40619742035813033No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATCGCGCATCGCGTATGCCG220000.3988370636382606No Hit
ATACACATCTCCGAGCCCACGAGACAACATCGCGCATCGCGTATGCCGT218870.39678849144775497No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA217550.39439546906592543No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA215290.3902983246849142No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211330.3831192575394255No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA195620.35463866540416605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192250.34852920674752547No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA183070.3318868238193471No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA170650.3093706586812235No Hit
ACACATCTCCGAGCCCACGAGACAACATCGCGCATCGCGTATGCCGTCTT162920.29535697458157006No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA154430.27996548971662083No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA146430.26546232376613865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT141950.2573405508338686No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA138350.2508141261561516No Hit
TCTCCGAGCCCACGAGACAACATCGCGCATCTCGTATGCCGTCTTCTGCTT134900.24455963584000615RNA PCR Primer, Index 18 (96% over 27bp)
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA119230.21615155953449913No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA118340.21453808232250798No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA113860.20641630939023797No Hit
TCTCCGAGCCCACGAGACAACATCGCGCATCGCGTATGCCGTCTTCTGCTT107580.19503132411910942TruSeq Adapter, Index 18 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG107250.19443306852365203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT106940.19387107084307087No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA106930.19385294188563273No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA106550.19316404150298486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT103790.18816044925006847No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA96670.17525263155413934No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA90240.16359571192143924No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA89200.16171030034787656No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT89170.16165591347556224No Hit
CATCTCCGAGCCCACGAGACAACATCGCGCATCTCGTATGCCGTCTTCTG87880.159317277966047Illumina PCR Primer Index 3 (95% over 23bp)
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA81830.14834925871599483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT81140.14709836065276574No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT80860.14659074984449885No Hit
CATCTCCGAGCCCACGAGACAACATCGCGCATCGCGTATGCCGTCTTCTG80240.1454667544833365TruSeq Adapter, Index 18 (95% over 23bp)
GTATCAACGCAGAGTACATGGGGTGGTATCNNNNN78710.14269302399530676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT78530.14236670276142094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT77910.14124270740025854No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA77660.14078948346430598No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA76650.1389584587630576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG68920.12494477466340417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTT67740.12280555768570806No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA63430.11499197702988576No Hit
ATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA63180.11453875309393322No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT62220.11279837317987533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG61780.11200069905259881No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT61040.1106591562021792No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA59680.10819361799059724No Hit
GATACCACCCCATGTACTCTGCGTTGATACNNNNN57820.10482163190711012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT57380.1040239577798336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT56860.10308125199305225No Hit
ACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA55450.10052506899427976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)