FastQCFastQC Report
Sun 15 May 2022
EGAF00006138758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11698945
Sequences flagged as poor quality0
Sequence length35-51
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2868422.451862112352866No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1862481.5920068006132178No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1630511.393723963998463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1399981.1966720076041044No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1344091.148898469049987No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1084320.926852805958144No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA886440.7577093490054018No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG833100.712115494174902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT764140.6531700080648297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT667580.5706326510638352No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA629020.5376724140510105No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA579400.49525833312320044No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA539290.46097319031758843No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA518830.4434844338527961No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT496640.42451691156766697No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA471200.40277136100733874No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA441520.377401552020289No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA412090.35224543751594695No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA405100.34627053977944167No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT373800.31951599054444657No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA371220.3173106634829038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT369050.31545579537300156No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA367390.31403686400782294No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA365560.3124726203944031No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA324480.2773583429958855No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA309970.26495551521953475No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA307370.2627330925993754No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA294090.2513816416779462No Hit
GAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA281560.24067127420463985No Hit
GAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA275130.23517505210939962No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA274940.23501264430254182No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT274740.23484168871637573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT271200.23181577484123567No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG265140.22663582058040274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT252360.21571175862438877No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATT252220.2155920897140725No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA251540.21501084072110777No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA248950.2127969658802567No Hit
GAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA242210.20703576262645906No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA241080.20606986356462054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT240430.20551425790958072No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA231360.19776142207694794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT230570.19708614751159184No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA228940.19569285948433812No Hit
ACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA226790.1938550869330525No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA226360.1934875324227954No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATT216280.1848713708800238No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT210270.1797341555157324No Hit
GGTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAA207700.177537376233498No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT206590.1765885727302761No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN206370.17640052158549338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT202490.173083983213871No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA194760.16647654980855112No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA187370.1601597408997136No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA170590.14581656722037756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG170530.14576528054452773No Hit
CATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA167640.14329497232442753No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT166210.1420726398833399No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT163080.13939718495984038No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA162930.13926896827021581No Hit
GTATCAACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA162850.1392005860357494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG159780.1365764177880997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT157550.13467026300234766No Hit
GAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156430.13371291171981747No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA151010.12908001533471608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT150770.12887486863131675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT148740.1271396694317308No Hit
ACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148390.12684049715594015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT145210.1241223033358991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT142800.12206228852259754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT137670.11767727773743701No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA132530.11328371917296816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT126150.10783023597426948No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATTA123760.10578731671958455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)