FastQCFastQC Report
Sat 28 May 2022
EGAF00006138765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006138765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11801151
Sequences flagged as poor quality0
Sequence length35-51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1556131.3186256154166658No Hit
CTTATACACATCTCCGAGCCCACGAGACACGCCTTGTTATCTCGTATGCCG1484741.2581315161546531No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1147610.9724559917926651No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1047460.887591388331528No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA998930.8464682809329361No Hit
ATACACATCTCCGAGCCCACGAGACACGCCTTGTTATCTCGTATGCCGT971700.8233942604412061Illumina PCR Primer Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACGCCTTGTTATCGCGTATGCCG770350.6527753097981713No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA763450.6469284224903147No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA709290.6010345939985007No Hit
ATACACATCTCCGAGCCCACGAGACACGCCTTGTTATCGCGTATGCCGT670890.5684953950678201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT649780.5506073094056674No Hit
ACACATCTCCGAGCCCACGAGACACGCCTTGTTATCTCGTATGCCGTCTT613800.5201187579075973Illumina PCR Primer Index 12 (95% over 24bp)
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA535900.45410824757686774No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA475060.4025539542710707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT462740.39211429461414404No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA441340.3739804702100668No Hit
ACACATCTCCGAGCCCACGAGACACGCCTTGTTATCGCGTATGCCGTCTT435120.36870979788327424No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA433120.367015047938968No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA402330.3409243725463728No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT376250.31882483327261896No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA338140.28653137308386273No Hit
TCTCCGAGCCCACGAGACACGCCTTGTTATCTCGTATGCCGTCTTCTGCTT326660.2768035084035447Illumina PCR Primer Index 12 (96% over 30bp)
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA312970.2652029450347682No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA307960.260957596424281No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA299280.25360238166599175No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT289920.24567095192663835No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA289580.24538284443610625No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA272390.23081646866479377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT269890.22869803123441093No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA268570.2275794962711688No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA268090.22717275628453532No Hit
TCTCCGAGCCCACGAGACACGCCTTGTTATCGCGTATGCCGTCTTCTGCTT252720.2141486029625415Illumina PCR Primer Index 10 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT252540.21399607546755398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT252380.21386049547200947No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT237410.20117529213887694No Hit
CATCTCCGAGCCCACGAGACACGCCTTGTTATCTCGTATGCCGTCTTCTG231290.19598935730929973Illumina PCR Primer Index 12 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT228830.193904814877803No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA226030.19153216495577421No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA217010.1838888427069529No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA215470.18258388524983707No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN211940.17959265159813648No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG209900.1778640066549441No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA204460.173254286806431No Hit
CATCTCCGAGCCCACGAGACACGCCTTGTTATCGCGTATGCCGTCTTCTG198510.16821240572211982Illumina PCR Primer Index 10 (95% over 22bp)
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA192420.16305189214170718No Hit
ACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA188670.15987423599613293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT188530.15975560350003148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT188380.15962849725420852No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA186830.15831506604737114No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA185000.1567643698483309No Hit
GGTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAA182210.15440019367602364No Hit
GAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA158020.1339021930996392No Hit
GAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA151640.12849594077730214No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA147000.12456412090651157No Hit
CACATCTCCGAGCCCACGAGACACGCCTTGTTATCTCGTATGCCGTCTT146810.12440311966180248Illumina PCR Primer Index 12 (95% over 24bp)
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA146340.1240048534248905No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATT142640.1208695660279239No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT139490.1182003348656415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT137220.11627679367885388No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA136520.11568363119834667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT134960.11436172624178778No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTT133530.11314998003160877No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA133410.11304829503495041No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATT132320.11212465631530348No Hit
GTATCAACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA131770.11165860008061924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG128780.10912494891388137No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA125830.1066251927460296No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT125530.10637098025438367No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA124870.10581171277276259No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA123980.1050575490475463No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA123650.10477791530673576No Hit
CACATCTCCGAGCCCACGAGACACGCCTTGTTATCGCGTATGCCGTCTT121810.10321874535797398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT121720.1031424816104802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG119330.10111725542703419No Hit
GAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA118830.10069356794095762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)