FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006139133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28933
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1000.3456261016831991No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC520.17972557287526356No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG520.17972557287526356No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA430.14861922372377562No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA410.14170670169011162No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT410.14170670169011162No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA390.13479417965644766No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC380.13133791863961566No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC380.13133791863961566No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT380.13133791863961566No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT350.12096913558911969No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT350.12096913558911969No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC340.1175128745722877No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT320.11060035253862373No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC310.10714409152179172No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG310.10714409152179172No Hit
ACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTT300.10368783050495974No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT300.10368783050495974No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA300.10368783050495974No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG300.10368783050495974No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT290.10023156948812774No Hit
GGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTTTAATCT290.10023156948812774No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC290.10023156948812774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)