FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006139134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28933
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1010.3490823627000311No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG450.1555317457574396No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC420.14516296270694365No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT400.13825044067327963No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT390.13479417965644766No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC380.13133791863961566No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT370.1278816576227837No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA360.12442539660595167No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT360.12442539660595167No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG350.12096913558911969No Hit
TCTCTATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCC340.1175128745722877No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC340.1175128745722877No Hit
GGGATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATT330.11405661355545571No Hit
GTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGT320.11060035253862373No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC320.11060035253862373No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC310.10714409152179172No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG310.10714409152179172No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC300.10368783050495974No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA300.10368783050495974No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA300.10368783050495974No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT300.10368783050495974No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT300.10368783050495974No Hit
GTATAAGAGATCAGGTTCGTCCTTTAGTGTTGTGTATGGTTATCATTTGT290.10023156948812774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)