FastQCFastQC Report
Fri 3 Feb 2023
EGAF00006139146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56796
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1300.2288893584055215No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG850.14965842664976406No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG820.14437636453271357No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT770.13557292767096274No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG740.13029086555391226No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT700.12324811606451158No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC670.1179660539474611No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG640.11268399183041058No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC630.11092330445806042No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA630.11092330445806042No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA610.1074019297133601No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG580.10211986759630959No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT570.10035918022395943No Hit
GTATTGATTGGTAGTATTGGTTATGGTTCATTGTCCGGAGAGTATATTGT570.10035918022395943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)