FastQCFastQC Report
Fri 3 Feb 2023
EGAF00006139147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81778
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1760.21521680647606936No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1190.14551590892416053No Hit
CATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACACCGC1090.13328768128347476No Hit
GTTAAGGGTAGGTAGGTTTGTTGGTATCCTAGTGGGTGAGGGGTGGCTTT1010.12350509917092617No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC1000.1222822764068576No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA980.11983663087872043No Hit
ATATTGTACGGTACCATAAATACTTGACCACCTGTAGTACATAAAAACCC960.1173909853505833No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA880.10760840323803468No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA860.10516275770989753No Hit
ATATACACCAACAAACAATGTTCAACCAGTAACTACTACTAATCAACGCC840.10271711218176038No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC840.10271711218176038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)