FastQCFastQC Report
Fri 3 Feb 2023
EGAF00006139148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81778
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1760.21521680647606936No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG1020.12472792193499475No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC1000.1222822764068576No Hit
GTTAAGGGTAGGTAGGTTTGTTGGTATCCTAGTGGGTGAGGGGTGGCTTT920.11249969429430898No Hit
CTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCCAAGGACA900.11005404876617184No Hit
CATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACACCGC870.1063855804739661No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG870.1063855804739661No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)