FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006139149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67544
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2810.41602510955821387No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA1030.15249318962454103No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA890.13176595996683643No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT880.13028544356271468No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT840.12436337794622765No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG770.11399976311737535No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT710.1051166646926448No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT700.10363614828852305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)