FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006139151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57621
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2800.4859339476926815No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA860.14925114107703788No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT670.11627705176932021No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT630.10933513823085333No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC620.10759965984623661No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT580.10065774630776973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)