FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006139154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50625
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1820.3595061728395062No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC630.12444444444444445No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT610.12049382716049382No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG590.11654320987654321No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG580.1145679012345679No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG560.11061728395061728No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC510.10074074074074074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)