FastQCFastQC Report
Fri 3 Feb 2023
EGAF00006139165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114258
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3300.2888200388594234No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT2360.20655008839643613No Hit
GACCATAGCAGTCCCCTTAACTTGTTTGTTTCTCTCACTTCCTTTCTTTG1890.1654151131649425No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG1560.13653310927900017TruSeq Adapter, Index 11 (96% over 28bp)
GTGGTGAGGTGCGGGGACAAAACATGGGCTCCAGGGACAGACAGAGAGGA1550.13565789704003223No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA1410.1234049256944809No Hit
TAATCACACCATGCCTGCCATCCACTCTCTTAAGTACTACTGGTTTTCTT1330.1164032277827373No Hit
CTCTTAAGTACTACTGGTTTTCTTTACAACATTGATGGGGGAGAGAAGCT1300.11377759106583347No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG1300.11377759106583347No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG1230.10765110539305782No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC1220.10677589315408988No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1220.10677589315408988TruSeq Adapter, Index 11 (95% over 21bp)
ACTTAAGAGAGTGGATGGCAGGCATGGTGTGATTATGTGTGAGAGCTCTT1200.10502546867615396No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT1150.10064940748131422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)