FastQCFastQC Report
Fri 3 Feb 2023
EGAF00006139166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114258
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3270.28619440214251957No Hit
GACCATAGCAGTCCCCTTAACTTGTTTGTTTCTCTCACTTCCTTTCTTTG2040.17854329674946173No Hit
CTCTTAAGTACTACTGGTTTTCTTTACAACATTGATGGGGGAGAGAAGCT1410.1234049256944809No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG1390.12165450121654502No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT1390.12165450121654502No Hit
TAATCACACCATGCCTGCCATCCACTCTCTTAAGTACTACTGGTTTTCTT1270.11115195434892962No Hit
GTGGTGAGGTGCGGGGACAAAACATGGGCTCCAGGGACAGACAGAGAGGA1260.11027674210996168No Hit
CTTAAGTACTACTGGTTTTCTTTACAACATTGATGGGGGAGAGAAGCTGG1190.10415025643718602No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1180.10327504419821806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)