FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006139169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32509
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1330.4091174751607247No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA580.17841213202497772No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC540.16610784705773785No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC510.15687963333230798No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT510.15687963333230798No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC500.153803562090498No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT480.1476514196068781No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC460.1414992771232582No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC450.1384232058814482No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT430.1322710633978283No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA420.12919499215601835No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC400.12304284967239842No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG390.11996677843058845No Hit
CCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAAC380.11689070718877849No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA360.11073856470515857No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT350.10766249346334861No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG340.10458642222153866No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT330.10151035097972869No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT330.10151035097972869No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)