FastQCFastQC Report
Wed 8 Feb 2023
EGAF00006139170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32509
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1350.41526961764434467No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA500.153803562090498No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG460.1414992771232582No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT450.1384232058814482No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC450.1384232058814482No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT440.13534713463963824No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA430.1322710633978283No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT420.12919499215601835No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA410.12611892091420837No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG370.11381463594696854No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT370.11381463594696854No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC360.11073856470515857No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC350.10766249346334861No Hit
TTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAATT350.10766249346334861No Hit
CCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAAC340.10458642222153866No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG340.10458642222153866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)