FastQCFastQC Report
Wed 8 May 2024
EGAF00006139837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26589712
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT5618822.1131556445590687No Hit
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5316101.9993071004304221No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG4368901.6430790976600274No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC3346721.258652218572356No Hit
AGGGACGCCATCTCTGCCAGGTTCTCCAGCAGCTCCTCTGGGCCCTGCAG3322161.249415563432955No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG2490180.9365201097326666No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG2176130.8184105190759494No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT2150780.8088767565440348No Hit
CCAGGCTCCCCAGGGCGACCAGCATCTCCATTAGGTCCTCGGACTCCAGC2001010.752550460117808No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC1754880.6599845835110963No Hit
TGTGACTCGTGCAGCCATCGACAGTGACGCTGTAGGTGAAGCGGCTGTTG1725640.6489878491350339No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG1704070.6408756890634995No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1584250.59581314758129No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT1506520.5665800366698218No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG1457120.5480014225050651No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1139170.42842509915113036No Hit
TGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGTCACATCCTGGC1071460.40296036301559035No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT1049740.39479179014800914No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG1024790.3854084617388861No Hit
ACATGCTCTGGCCGAGCCAGTCGCTCTCTTTGATGGTCAGTGTGCTGGTC906560.34094389589477314No Hit
GATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACACTTACAT850200.3197477279934435No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC819950.30837114745733235No Hit
ATGGCCTGCACCTCGCCGCGGTACTGCACCAGGCGGCCGCACACGTCCTC802790.3019175235895748No Hit
CCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGA798150.3001724877651928No Hit
CATTCAGGATCGCAGACCAGCTCATCAGCGAGGCAGCCACCGAGTTGGCC794930.2989614930767208No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC790820.2974157824650376No Hit
GATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGG784480.2950314016187915No Hit
TCCATGCCCAGTAGTGAGTTCTTGTCGGTGGCCTGCAGGAAGACGTTCTC769570.2894239696917364No Hit
GGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCTCCCAGGCTGGA759190.28552020420529567No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC754290.2836773862010991No Hit
AAGCGAGGCGCACGAGTACGAGCGAAGTCTGGTCTGCGCAGTGGCCACCA753930.28354199549058673No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG747580.28115385379127084No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG744190.2798789246006125No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG727120.27345914841048297No Hit
GGGTTCTCCCTTCTGCCCTTTGGGTCCTCGGATCCCGGGAATGGCTGGGA698200.2625827613326538No Hit
CAGCCCATCATTTCCAGGAGGACATGGCATTTCTCCAGGTGGACCCATGG695850.26169896086125344No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC648980.24407184252315334No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC646300.2430639339004499No Hit
CACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACA627430.23596720415775843No Hit
CTTCGCACGCAGCACCAGAGGCTGCCATCAAGATGAGGGTGAGGGCCAGA615920.23163846227443158No Hit
GAGAGTCTCAGGGATCCCCCAGGCTGTACCAAGCCTCCCCCAGACTCCAC612990.23053653232498342No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC593240.22310884751215054No Hit
GGTCAATCCGGAGAGTCCAGTTGCTCCCAGTGACTGCAGAGTAGCCACAA585350.2201415344400872No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA581110.21854693273849674No Hit
CGTGTGTTGTAACCACTCTCCCATTTGGCCAAACACATCCAGTTTGCTAG570550.21457547189679976No Hit
CTTCATGGCTGTGAAATTCATGGCTGTGGAATTCACGGCTGACTTTGGAA568250.21371047569074836No Hit
GCACTGGGATGACGATGAGCAGAATGGTCATGAGGATGCCCAGAATCAGG524800.19736956910251605No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT524720.19733948227795775No Hit
TGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCT518480.19499270996240953No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT517270.19453764674096508No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT514370.19344699935072632No Hit
ACCTGCTCTTGTCCACGGTGAGCCTGCTGTAGAGGAAGAAGGAGCCGTCG485320.18252172118298987No Hit
TATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCTGAATCTCATC483910.18199144090014965No Hit
TCCGGTGGTATCTGGGAGCCAGAGTAGCAGGAGGAAGAGAAGCTGTGCTG469590.1766058993042121No Hit
CAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAG469260.17648179115290907No Hit
TTTGTGAAACTTGTTTCCTGCAGGTGATGGTTCATGACAATATCGACACC467210.17571081627360238No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG454390.17088940263813313No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT446160.16779422056169693No Hit
AAACCTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCCCCAGACTGTACC438750.16500742843698343No Hit
GGTTTGCACCGCCCCTGTGTCATATTCCGGCGCCTCATCATTTGGTTACA433090.162878785599483No Hit
GCTTTCAGGGCAGTTACATTCTGGTGCACAGTTTGGTGATGATTGCCCAT430180.16178437735617446No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG421750.1586139782183425No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC417300.15694039860228648No Hit
GTGCACAGGAGCAGAGGGCCATGGTGCAGACGAGGACAAGGAGGGCAGCT402000.15118629340550963No Hit
GTGATGTTGGCGGTTAGTGGGGTCTTCAACTCGGGGTGGGCAGCAGTGCA400370.15057327435513404No Hit
TAGTTGGAGCGCTTTGTCATGATTTCCAGGTTGGCTTTGTCCACAGCTAT393030.14781280820190906No Hit
CATGCTGGAGATTGTCCTGAGTCCTGTCTTCTCTACCCACAGCTGAAGCT381530.14348782717165195No Hit
TGGTGCAGACCACACCAGCGTCTCTCTCGTGCCTGCAGTTGCTCTTCAGC380740.14319071977913864No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC377460.1419571599722479No Hit
GTGCTTCATAGTAGAGGGTCACATTGACAAGCGGTGCATTGGACTTCTTC366630.13788415609766663No Hit
CAGCAGGAGCCCCAGGAGCTGAGCGGGGACCATCATGTCCATGCTGTGTC363620.1367521393236602No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC363580.13673709591138106No Hit
ACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCCACCCAT350990.13200218189651697No Hit
CCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTG350170.1316937919447943No Hit
GGCTCCACCTCAAGGGTGATGGTCTTGCCGGTAAGGGTTTTCACGAAGAT346170.13018945071687876No Hit
AGGAGCTGCAGAGGGTCAGGATGCTCAGGCAGAAGTCCAGCAGCTGCAGC340430.12803072105482No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA338780.12741018029830484No Hit
GGTGCTCCGCCTCAGAGGCATCATTTGACTTTTGCTCGGAGATCTGAGCC338560.1273274415307695No Hit
GTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCAC333860.12555984058796876No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA333040.12525145063624607No Hit
CGGCTGCGCTTGCGCTTCTTGCCATCTTTCTTCTGCGCTTTGGTCACTGC303230.11404034763520567No Hit
TGCAAATCCAGCAAGAAGTCCCAGTCTTTTCTGTTCAGTTTCATGGCTAT294790.11086618764430393No Hit
TCCCACCGGTGGACTTACGAGCAGTCTGCTTGGTTCGGGCCATTTTCTTT293950.11055027598644168No Hit
CAGCACCAGTTCAAGAAGAGGGTGTTGAACAGGGACCAGACGACATGGTC287860.10825991646694029No Hit
GGCGTTGTCTCTGGAGATGGTGAATCGGCCCTTCACTGAGTCTGCGTAGT287270.10803802613582275No Hit
CCTGCACACCACACCCACAGTGGTTTCAGACATGCTACTCTTGCCAACAG286070.10758672376744811No Hit
GCTTTGGCCATGCCGGTTCCCTGCTCTCTGTCGGCTCCGCTCTGAGATTG285440.10734979002405141No Hit
CTGACTCCCCAAAGACATGCCTTCTCTCTGAGTGCCCCAGGACCACCCAC280420.10546184178301743No Hit
AGCTCCAGGATCTCGGCGGTCAGGTACTCGAGGACCGCCGCCATGTAGAC278250.10464573666687327No Hit
CTGCGCTTGCGCTTCTTGCCGTCCTTCTTCTGCACTTTCGTAACAGCCTT276320.10391989202440403No Hit
TTAAACCCATTAACATGCAGGATCCTCATCTGCTTCACAATGGTGCTTTT274500.10323541676570247No Hit
ACTTGCACATGGACAACGCCGCCTTCTTCTCCCGGAGCCAGCTCGAGTTG273910.10301352643458492No Hit
CCATCAGGAGTCTGCCAGGTGACTTCACACGTGTAGTGGCTCCGGTCATC271880.1022500732614178No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG269600.10139259876150596No Hit
TGCAGCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACC269020.1011744692834582No Hit
AGGTTGTATCTGGCGCCCTTCCCATCCTTATAGGTGCTGGTCACGATCCT267360.10055016767387326No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)