FastQCFastQC Report
Fri 10 May 2024
EGAF00006139839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38336269
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT6894011.7982996728241865No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA4226351.1024416590983332No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC3034720.7916054637450505No Hit
TGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGTCACATCCTGGC2892930.7546196005667636No Hit
AGGGACGCCATCTCTGCCAGGTTCTCCAGCAGCTCCTCTGGGCCCTGCAG2663290.6947181010233416No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG2540150.6625970826738512No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC2178190.5681799655568985No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1824970.4760426738449691No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC1820640.47491319512600455No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG1771360.4620585273960802No Hit
CCAGGCTCCCCAGGGCGACCAGCATCTCCATTAGGTCCTCGGACTCCAGC1760080.4591161440358215No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC1477710.38546004568154507No Hit
CAGCCCATCATTTCCAGGAGGACATGGCATTTCTCCAGGTGGACCCATGG1365460.3561796793527299No Hit
GCTTTGGCCATGCCGGTTCCCTGCTCTCTGTCGGCTCCGCTCTGAGATTG1361170.3550606346173124No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG1350020.35215216170358155No Hit
TTCTCCTCGGAGCAGGGCGGGCAGTGGATGGCTTCGTCGCCCAGGCTCGG1347530.35150264622777977No Hit
AGGAGCCTGCGCGGGACCCCAGCATCCTGAGGCTGCCCAGGGTCGTCGGG1338920.34925673126928447No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG1287890.3359455767591781No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1270040.33128941160132197No Hit
TTGCTGTTCTGGTCATGGGCCGAGTTGTAGTTGGTCACGACGCGGGTAGG1221220.31855473468218826No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA1213370.3165070654110863No Hit
ACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCCACCCAT1160510.30271855615370397No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC1151110.30026657002015505No Hit
TCATTGCACACTCCTGGGAGCTCCTTCAGCTTTGTCTCTGATTCCCTGGT1140080.29738939905706524No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC1111400.28990823285385436No Hit
GCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGG1109470.28940479314771084No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC1099770.28687455213755936No Hit
CAGCAGGAGCCCCAGGAGCTGAGCGGGGACCATCATGTCCATGCTGTGTC1093000.2851086004222268No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG1056010.2754597741371233No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA1026990.26788991907376275No Hit
TCGGCCACGACCCCTCGTACCACCTCGTCCACCAGCTGCAGCACCTCTTC983840.2566342593224187No Hit
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT980900.25586736153171297No Hit
ACGCTGTGGCAGCTCTTGCCCTGCTCCACGCAGACGTCCAGCAGCGCCCC948480.24741061786685606No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG912620.23805655161695574No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC911770.23783482946658163No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT907230.23665057233399528No Hit
TCCCACCGGTGGACTTACGAGCAGTCTGCTTGGTTCGGGCCATTTTCTTT896380.233820354296867No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG840610.2192727727364392No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT837910.2185684788470156No Hit
GGTCATACTCCTCCACCTGGCCGCTGGTCCACACCTTCACGCGGTCTGTG814590.212485466439105No Hit
GTATGTCACTGCAGTCGGCGGCAGTGGCTGGGCGGATCACGAATTTAGCC762110.19879608002541926No Hit
AGGAGGACCCGGGAGCCCATCTCTTCCACTTGGTCCTGGCAGAGATGTAC741540.19343040398636602No Hit
CAGCACCTCTCCCTGGCCAGCACTTCTGGTCTCCACAGTGAACTCTGCCC741100.19331563016734885No Hit
TGCACCTGGCCCCGAAGCTTGTTGTGCAGCATGAGGATCTCCTCCTTGTC732890.1911740550443237No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT725370.18921246613748458No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG724050.18886814468043303No Hit
GCCGCGTCCTCCTTGCCCAGAAGGTAGGCCTTCACGGTGAGCGACGCCAT675680.17625085007620328No Hit
GTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCAC668810.1744588134020032No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC657140.17141469870216114No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT640130.16697764719879235No Hit
GCTTCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTT620370.1618232593265662No Hit
ATTCCTCGGGCGGAGTCCCACACGTGTTGGTGGCCACCACAGTCACGTTG607960.1585861159311043No Hit
CTGACTCCCCAAAGACATGCCTTCTCTCTGAGTGCCCCAGGACCACCCAC601840.15698971644841078No Hit
CCAGCACGGCGTCCGACTCCTCGGTGATCTCCAGCAGCGCGTGCACGTCG599780.15645236629573941No Hit
ATGACCTCACAGCTCAAGTACACCTGGGCACCAGTGACATTCCAGATGTC599010.15625151211245936No Hit
CAAGCTGGGCCCGCGACTCGAAGGTGACTGGGATGGTGATCTCGTTGGAC591170.1542064513372441No Hit
GCACTGGGATGACGATGAGCAGAATGGTCATGAGGATGCCCAGAATCAGG587550.1532621758262391No Hit
AGGTCCCTGTGCACGATGGGCATCTTGGGCCTCCCACATGGAGTGTGATC585820.15281090603783065No Hit
GCCTCGTCAGATCCGTCTGGGCAGTCCCTCTCACCGTCGCACCGCCAGCC577040.15052064664926051No Hit
ACCTTGCTGGGCTCCTCGGCGGCCTTGGTCTCGTCGCTGGCGGGCGGCTT556300.14511062618013246No Hit
GGTTTGCACCGCCCCTGTGTCATATTCCGGCGCCTCATCATTTGGTTACA550080.14348814173857138No Hit
GAAGGTCCTCTCGTACCTCCCCGCTGTCCTGGAGCAGTGATAGGTACCCA545330.14224910619236317No Hit
AAGCGAGGCGCACGAGTACGAGCGAAGTCTGGTCTGCGCAGTGGCCACCA542020.14138569405384754No Hit
GGGTTCTCCCTTCTGCCCTTTGGGTCCTCGGATCCCGGGAATGGCTGGGA539570.1407466125615928No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT535640.1397214736780984No Hit
CGGCCAGGCACACGAAGTGCGGCATCACCGCCTGGTAACTGGAGCAGATG528810.13793987098744534No Hit
GCCCAGTGCCAGCTGGTAGTGGTCTACCTCACCGAGGAGGCGGAAGGTCG528310.13780944619310764No Hit
AGCGGAGGTCCTCCACGCCGTCATTCACCATGTCCACCAGGGCTGCCTCC514470.1341992878858399No Hit
ACCTCCTGCCAGATCTCCGTGACCTTGGCGTACATGTCTCGGATCCACTC502140.13098301245747207No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG501870.1309125830685297No Hit
AAGACAGCCAGGACGAGCAGCAGCCCGAACAGGACAGACCCTCCTTGGAT501080.13070651189347612No Hit
AACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCT500810.13063608250453376No Hit
GTCACCTCGGTCCCTTCTGAGACCTCTGGCTTCGTCAGAATCACGTTGGG500060.1304404453130272No Hit
CTCCTCCGAGCGCTCCAGCTCTCCTTCCAGGATCACCAGCTTCCTGGCCA499210.1302187231626531No Hit
GCTGGTGGCCCCAGACCTGCCCATTGGCTTTGTGTCCTTGGCGCCGGTGG498050.12991613763978963No Hit
AACCCCACTCTGCTTCAGGTTACTTGCTGGCCCATCCACACGGTCAGATG497760.12984049125907376No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA493290.12867449359769467No Hit
TGTGACTCGTGCAGCCATCGACAGTGACGCTGTAGGTGAAGCGGCTGTTG486180.12681985302221246No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG478070.12470436285805488No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC476380.1242635270531934No Hit
AGTTGTGCTCCCTCTGGGACTCCATCCTTCATCTCTCCAATCTCACCAGC474930.12388529514961406No Hit
CTTCGCACGCAGCACCAGAGGCTGCCATCAAGATGAGGGTGAGGGCCAGA474450.12376008734704985No Hit
GCGTCATCATCCACCACGGCCTGCTGTAGGGCCTGGTCATAGAACTGCTT471140.12289667520853426No Hit
TTCCTGCTTCATGCGGGCCAGCTCTGTGCGGACATGCTCGTCGCTGCGCA470620.12276103342242303No Hit
TGGGAGGCTCAGGAGCTGGTTGTTTAGCTTCTCGGGCACGACGCTGAGGA466910.12179328144843725No Hit
GGCGGCTGCTGCTAGGGAGTCGACTTCTCTCCGGTGGCGACTCCGCTTTT461240.12031426428064766No Hit
CCCGCACCGTGGAATGCGGCCATCTTGTCATCTGCCACCACCAGTGTCTC460730.12018123099042319No Hit
GAAGGACGCCGCCCTGAGCAATGGTCACCCGGCCTAGCAGTTTGTTGAGC460660.12016297151921593No Hit
ACGCCGATGCCCCATTTTCTGTCTTCTGCCGTTTGGGATCCGCTTCATCC460280.12006384867551925No Hit
TCTTTGCCACTCCTGCCCCATTCATTGGCAGCCTGATCAGCCAGCGAGTC456970.11920043653700366No Hit
GGAGCTGAGCGAGGCGCGGGAGGCTGTGGCTGGGAGCGGCGCTGAGAGCT452100.11793009904015439No Hit
CATCCAGGATTCGGGTCACCTCGCTCTGCTGGGACTTGATTTCGCGGGAG444800.11602589704282386No Hit
ACACTCATCTGGACAACCCCTTCTTCCCAGCCTCGGATCACCTCCTGCTT442990.11555375928732135No Hit
CGCGCCGGATCTTGTACTCGTCGCTGTGCTTGTCCACGGTCTTCTTGGCC442470.11541811750121014No Hit
TGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCT439130.11454687987503427No Hit
CTGAGGAGCTGGTTCTGGTGGAACTTGGAGCTGGGCAGGGTGACTGAGTG434920.11344870310671076No Hit
ACTTGCACATGGACAACGCCGCCTTCTTCTCCCGGAGCCAGCTCGAGTTG429140.11194099248416688No Hit
GTCTTCACACCGTTCTCTGCCATCTCACACACAGACTTCAGGTAGGGATA423880.1105689236477342No Hit
CGGTTTGCTGCCCTCTCTCCGTCATCTGCGCTGAGGAGTTGTCCCGGGTG422730.1102689466207575No Hit
CGATCTTGACTTGCTCGCGGTAGCTCTTTTCGTTGAGGCAAACCCCGCGG421800.11002635650328935No Hit
ACGTCATCCGTTGCCAGGTCTTCCTGCATGAGCTCACCAGGAACCTCTTC417860.10899860912390823No Hit
TCGATCATAGTCTTCCTGCCCTGCTGTGTCCCACAGAGCCAGCTCCACCT417330.10886035884191025No Hit
AGCTCCTTGGTCAGGCTCTGCACCTGTTCCTCCAGCTGCTGCACCTTCTC414810.10820301787844822No Hit
CCCAGCCGGCCACGTCGCAGAGAGTTCCCGGTGCCACGTCGCGGTCCACG412860.10769436118053116No Hit
CGGGCTCCGGGAGAGGGTCGAAGGTGAAGATCTCAGGACCGGAGCCCCGC409870.10691442091039166No Hit
GCACACTCATACTCATCACAGCACTGGGTCTTCCGAAGGGTGGGCAAACG394810.10298602610493994No Hit
CTCTCGTCGTCCTTGTCCAGCTCCTGGATCTCCTGGATGCTCTTCTGGGC394470.1028973372447903No Hit
CCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGA394310.10285560131060224No Hit
GCTTTCAGGGCAGTTACATTCTGGTGCACAGTTTGGTGATGATTGCCCAT392390.10235477010034544No Hit
CCACTTCTTGCTGTCGCCGCTCAATCTTTTCCCGTCTTTTCCTCTCCATG388150.1012487678443617No Hit
TGGTGCAGACCACACCAGCGTCTCTCTCGTGCCTGCAGTTGCTCTTCAGC385390.10052882297961756No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)