FastQCFastQC Report
Thu 9 May 2024
EGAF00006139841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85203656
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT9447891.1088596949407898No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA7362360.8640896817854858No Hit
TTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCT7284310.8549292767437116No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT4961550.5823165616273556No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG3743850.43940015907298624No Hit
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG3633270.4264218427434617No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC3481200.4085740170586107No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA3474210.4077536297268747No Hit
GGAAACGGAACACTGGATCCGCGAGAAGAGCTCGGTCCTTCCGGACGCCG3371740.3957271504875331No Hit
TGATCTATCCATAGGCCTGGAAGATCAGCACTGGGATGACGATGAGCAGA3185860.3739111852195638No Hit
TCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGATTC3005940.35279472045190174No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG2947070.3458853925235321No Hit
TCATCCCTCAGCCAGAGATTAGTCTACTTCTTCCATGCGTGATGTGTCGT2927720.34361436321464894No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT2870940.3369503299248098No Hit
CCAGTAATCTGCCCAGGGCCTCACCACCAACTGCATCCACGTTCACTTTG2816550.3305668010302281No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT2609350.3062485957175359No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC2466440.2894758412714121No Hit
GATGCCGACCACCGTGCCCACGTCCTCCTTCTTGTCCTCCTCCTCGGCCC2444490.28689966073756273No Hit
TTGCTGTTCTGGTCATGGGCCGAGTTGTAGTTGGTCACGACGCGGGTAGG2265580.2659017354842144No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG2143000.25151502888561494No Hit
GTGGGCATCCGGGCTAAGGCTTTACTTGGCGGCAGTCTCATCGGATTTTG2134920.2505667127711046No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG2072550.24324660434758807No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC1954210.22935752897739503No Hit
GAATAATTCTGACGAATCTGGGAGCTCATGGTTGGTTGGCAAGAAGGAGC1921500.22551849183560854No Hit
ACATAGGGACAGTTGTCCAGATTGTTCTGGTGGCCATCTTCATCAATATC1731500.2032189792419236No Hit
GCCCATTCACGTAAAAACTTGACCGGTCTTTGCCAACCAGGACACCCACC1616680.18974303168399254No Hit
GGGTAACCGAAATTGGAGAGCATGTCGGTCCAGAGACACTCGTTCTTGGA1583590.18585939551701866No Hit
AGCAAAGTCCTTGAGTCCCAACAGTCCACCTCAAAGACAAACATACTAAA1519950.17839023245669175No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA1482950.17404769579371102No Hit
TTCTTTGATGTGAAAGGGGCAGCACAGTCATTTAAACTTGATCCAACCTC1481730.1739045094496884No Hit
TAGACACGGACTCGGTAGTTGGACTTGGTCTCCTTTCCATTCCTGTTCAT1477130.17336462651320972No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC1441250.16915353960867593No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT1428080.16760783128836632No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC1414770.1660456917482508No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG1406780.16510793856075848No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT1395350.16376644682946467No Hit
TGCTACATCTACACTTGGTTGGCTTAATCAACCTCTTCAATGGTGGGCCC1378690.16181113167256578No Hit
GGCGGCTGCTGCTAGGGAGTCGACTTCTCTCCGGTGGCGACTCCGCTTTT1330750.1561846125476118No Hit
GTGTGACCACACTTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAAGGCG1309580.15369997738125227No Hit
GAGTGAGCAGACACTCGGGAGGTGTCTTGAGTTTATCATCCGCTGAGGGT1291510.15157917636773707No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG1283560.150646117814475No Hit
TCATTGCACACTCCTGGGAGCTCCTTCAGCTTTGTCTCTGATTCCCTGGT1280200.15025176853913405No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG1259980.14787863093574294No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC1227770.14409827672183456No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA1222040.14342577036835133No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC1215370.14264294011045725No Hit
TGTAGGAGTCGGTGGCCAGGAGGATGTTGAACTCGGCTTCTGCCGCAGGA1212390.14229318986030365No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG1203390.14123689715849752No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT1194070.14014304738284938No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1163880.1365997721975686No Hit
GCTTGTGGGAGATTTTCATCTTCTTGGTACAGCCGCTGTAGATCTCTTCA1150170.1349906863151506No Hit
GCAAGGAGATGGCAACAGCCTTCTTCTGCATCTTCCCCATCCAGATCTTC1132300.13289335847278666No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1131700.13282293895933292No Hit
ACGTCATCCGTTGCCAGGTCTTCCTGCATGAGCTCACCAGGAACCTCTTC1131020.13274313017741868No Hit
TATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCTGAATCTCATC1119900.13143802186140932No Hit
TGGCACCAAACACCACTCCAGTCTTCTTAATGTCTCTCCAGTACAGGAGG1108140.130057799397716No Hit
CTGTTGAAGAGGTCGGCGAAGAGCTCGTCGTGGCACAGCTCCAGGGTGGG1099750.12907309986792115No Hit
GTCACCTCGGTCCCTTCTGAGACCTCTGGCTTCGTCAGAATCACGTTGGG1086590.1275285652061691No Hit
GGTGCTCCGCCTCAGAGGCATCATTTGACTTTTGCTCGGAGATCTGAGCC1072000.12581619737068558No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG1061860.12462610759331737No Hit
GCAGCAGAAAGCCCGTCATCTCGGCGCTTTCCCTTAACCGAGTCGCTCTG1045250.12267666072920627No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC1013340.11893151627202476No Hit
TACCAGAGCTGCTCCTTTTACTGCTTCCACCAGCCAGGGCTCTAGAGGTC1010110.1185524245579321No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC975700.11451386546136001No Hit
TGTCAATTTCTGGGTGGATCCAAGTGCCCTTGTAATCTGGGTTGTCGATC972520.11414064204005518No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG969710.11381084398538015No Hit
CGTGTGTTGTAACCACTCTCCCATTTGGCCAAACACATCCAGTTTGCTAG967170.11351273471175932No Hit
GCTTCCTGCGTCTCAAAGTGTACAAATCCATAGCCCTTGGAACCATTTTC966700.11345757275955388No Hit
ACCAAAGATCACCCGGCCTACATCTTCATCTCCAATTCGTAGGTCAAAAT964170.11316063714449061No Hit
GAGGATTGGGGTTCAGAGCACTTCTGGGGTGTCAGCTCCTCCTTGTCCCC955720.1121688956633504No Hit
AGCCTCTGGATGGCCCGGTTCATCTCTGAAATCTCATTCCGGGTATTCCG940950.11043540197383078No Hit
GGCGTTGCCGCCTTTGCCACAAGATGACCCCAATGAGCAGGGCGGCTGTC917810.10771955607163147No Hit
CCAACTTCGGATTCCGAGTTCAGCTCCAACTGAAGGTCCCTGATGTAGTC909310.10672194629770346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG903770.1060717394568139No Hit
TTCGAGGCAAGGAGAAGCAGGAGTTCCCGATCAAGAGGAAAACACGCAGC893490.10486521845963981No Hit
ATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAAAGGGTGTAACGCAA893070.1048159248002222No Hit
TTCCCCGCGGCCTTCGCTTTTAGCTCCTCGAGTTTCTTCTGCTCCTCTTT882600.1035871042904544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)