FastQCFastQC Report
Thu 9 May 2024
EGAF00006139847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85172628
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT9479411.1129643669090497No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA7360240.8641555594597833No Hit
TTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCT7277380.8544270819024159No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT4961830.582561571306688No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG3746900.4399183268126938No Hit
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG3633600.42661593111815216No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC3486040.40929111639011534No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA3483450.4089870280860654No Hit
GGAAACGGAACACTGGATCCGCGAGAAGAGCTCGGTCCTTCCGGACGCCG3383120.3972074220840057No Hit
TGATCTATCCATAGGCCTGGAAGATCAGCACTGGGATGACGATGAGCAGA3191320.37468845037868265No Hit
TCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGATTC2999370.35215186738161935No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG2949940.34634835971011724No Hit
TCATCCCTCAGCCAGAGATTAGTCTACTTCTTCCATGCGTGATGTGTCGT2942220.34544196522854737No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT2882270.33840331896298886No Hit
CCAGTAATCTGCCCAGGGCCTCACCACCAACTGCATCCACGTTCACTTTG2806270.32948026448121337No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT2608760.3062908896036412No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC2452260.28791644188787974No Hit
GATGCCGACCACCGTGCCCACGTCCTCCTTCTTGTCCTCCTCCTCGGCCC2439760.2864488342428509No Hit
TTGCTGTTCTGGTCATGGGCCGAGTTGTAGTTGGTCACGACGCGGGTAGG2268590.26635200219488353No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG2154570.2529650722999882No Hit
GTGGGCATCCGGGCTAAGGCTTTACTTGGCGGCAGTCTCATCGGATTTTG2134960.2506626894264669No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG2075970.24373675542804668No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC1952060.2291886543644045No Hit
GAATAATTCTGACGAATCTGGGAGCTCATGGTTGGTTGGCAAGAAGGAGC1904700.22362818251891908No Hit
ACATAGGGACAGTTGTCCAGATTGTTCTGGTGGCCATCTTCATCAATATC1727930.20287386224597886No Hit
GCCCATTCACGTAAAAACTTGACCGGTCTTTGCCAACCAGGACACCCACC1609690.18899146801012173No Hit
GGGTAACCGAAATTGGAGAGCATGTCGGTCCAGAGACACTCGTTCTTGGA1591310.1868334977288713No Hit
AGCAAAGTCCTTGAGTCCCAACAGTCCACCTCAAAGACAAACATACTAAA1506100.1768291099342385No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA1490530.17500105785159054No Hit
TTCTTTGATGTGAAAGGGGCAGCACAGTCATTTAAACTTGATCCAACCTC1477280.17344539374785992No Hit
TAGACACGGACTCGGTAGTTGGACTTGGTCTCCTTTCCATTCCTGTTCAT1468140.17237227903781482No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC1444870.16964018064582906No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG1413470.16595355024151656No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT1406740.165163390285433No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC1406450.16512934178806835No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT1393740.16363707833460298No Hit
TGCTACATCTACACTTGGTTGGCTTAATCAACCTCTTCAATGGTGGGCCC1367310.16053396872995396No Hit
GGCGGCTGCTGCTAGGGAGTCGACTTCTCTCCGGTGGCGACTCCGCTTTT1330170.15617341289504416No Hit
GTGTGACCACACTTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAAGGCG1308640.15364560548724646No Hit
GAGTGAGCAGACACTCGGGAGGTGTCTTGAGTTTATCATCCGCTGAGGGT1290950.15156864714800158No Hit
TCATTGCACACTCCTGGGAGCTCCTTCAGCTTTGTCTCTGATTCCCTGGT1285260.15090059214798443No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG1282090.15052840684920513No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG1262740.1482565502147004No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC1229320.14433275441495125No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA1227620.1441331597752273No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC1219070.14312931614602756No Hit
TGTAGGAGTCGGTGGCCAGGAGGATGTTGAACTCGGCTTCTGCCGCAGGA1211270.14221352897552955No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG1198200.14067899842188739No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT1192990.14006729955543934No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1170380.1374126908471111No Hit
GCTTGTGGGAGATTTTCATCTTCTTGGTACAGCCGCTGTAGATCTCTTCA1151630.1352112793795678No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1137110.13350650633910227No Hit
GCAAGGAGATGGCAACAGCCTTCTTCTGCATCTTCCCCATCCAGATCTTC1129240.1325825005657921No Hit
ACGTCATCCGTTGCCAGGTCTTCCTGCATGAGCTCACCAGGAACCTCTTC1127930.13242869528459306No Hit
TATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCTGAATCTCATC1110200.13034704060088412No Hit
TGGCACCAAACACCACTCCAGTCTTCTTAATGTCTCTCCAGTACAGGAGG1107850.1300711303636187No Hit
GTCACCTCGGTCCCTTCTGAGACCTCTGGCTTCGTCAGAATCACGTTGGG1090940.12808575074142364No Hit
CTGTTGAAGAGGTCGGCGAAGAGCTCGTCGTGGCACAGCTCCAGGGTGGG1090940.12808575074142364No Hit
GGTGCTCCGCCTCAGAGGCATCATTTGACTTTTGCTCGGAGATCTGAGCC1075830.12631170662011276No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG1063100.12481709499441533No Hit
GCAGCAGAAAGCCCGTCATCTCGGCGCTTTCCCTTAACCGAGTCGCTCTG1044400.12262155395745214No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC1016940.11939751348285274No Hit
TACCAGAGCTGCTCCTTTTACTGCTTCCACCAGCCAGGGCTCTAGAGGTC1015930.1192789307851344No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG974550.11442056243703082No Hit
TGTCAATTTCTGGGTGGATCCAAGTGCCCTTGTAATCTGGGTTGTCGATC970790.11397910605740615No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC970210.1139110090626768No Hit
CGTGTGTTGTAACCACTCTCCCATTTGGCCAAACACATCCAGTTTGCTAG969070.11377716324545016No Hit
ACCAAAGATCACCCGGCCTACATCTTCATCTCCAATTCGTAGGTCAAAAT967170.11355408688340579No Hit
GCTTCCTGCGTCTCAAAGTGTACAAATCCATAGCCCTTGGAACCATTTTC963810.11315959394842202No Hit
GAGGATTGGGGTTCAGAGCACTTCTGGGGTGTCAGCTCCTCCTTGTCCCC952410.1118211357761557No Hit
AGCCTCTGGATGGCCCGGTTCATCTCTGAAATCTCATTCCGGGTATTCCG937310.11004826574096081No Hit
GGCGTTGCCGCCTTTGCCACAAGATGACCCCAATGAGCAGGGCGGCTGTC915590.10749815069695866No Hit
CCAACTTCGGATTCCGAGTTCAGCTCCAACTGAAGGTCCCTGATGTAGTC913670.10727272616268221No Hit
TTCGAGGCAAGGAGAAGCAGGAGTTCCCGATCAAGAGGAAAACACGCAGC896190.10522042363187384No Hit
TTCCCCGCGGCCTTCGCTTTTAGCTCCTCGAGTTTCTTCTGCTCCTCTTT892510.10478835994117734No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG888450.10431168097807196No Hit
ATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAAAGGGTGTAACGCAA888080.10426823979177913No Hit
CGCGCCGGATCTTGTACTCGTCGCTGTGCTTGTCCACGGTCTTCTTGGCC858100.10074832961594188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)