FastQCFastQC Report
Thu 9 May 2024
EGAF00006139849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78829611
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG6620230.8398151298754982No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG5465030.6932712125142924No Hit
AACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCT5457510.6923172562655422No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG5414080.6868079052172413No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC4943920.627165342728889No Hit
TGGGCTCAACTTTCTTGTCCACCTGGGTGTTGCTGGGCTTGTGATTCACG4575770.5804633489818947No Hit
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT4121830.5228783889343308No Hit
ATGTCTCGATCCCACTTAACTATCGTGGGCTGTGACAAAGTCACATGGTT4041110.5126385819663628No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG3951330.5012494606880655No Hit
CTGACCTCAAGTGATCCGCCCGCCACGGCCTCCCAAAGTGCTGGGATTGT3547000.4499578210528021No Hit
CTGCCCACTCAGACTTTATTCAAACACCAGGAAGGGCCGGTGCAAGGAGG3540520.4491357949235599No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC3350700.4250560109956651No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG3309800.4198676053342443No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT3292710.41769963827425205No Hit
GCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGG3208210.4069803160642262No Hit
TGTGGGACTTCCACTGCTCAGGCGACAGGCTCAGATAGCTGCTGGCCGCG3172220.40241477279394416No Hit
GGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCTCCCAGGCTGGA2839110.3601578092272966No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT2828670.3588334337968508No Hit
TGGGCTCAACTTTCTTGTCCACCTCGGTGTTGCTGGGCTTGTGATTCACG2688030.3409924222510752No Hit
GGCAGCTGTAGCTTTTGTGGGACTACCACTGCTCAGGCGTCAGGCTCAGG2499390.3170623282664683No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT2489610.31582167771955644No Hit
GTGTAGGAGGCCTGCAGGTACAAAATGACCAGGCTGTTGACGGCTGCCTC2415780.3064559077933291No Hit
TGGGCTCAACTTTCTTGTCCACCTAGGTGTTGCTGGGCTTGTGATTCACG2327560.2952646816942938No Hit
GATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACACTTACAT2167270.27493095202512163No Hit
GTGGGGAACGAGCGCCGGGTTCCGACCAAGCACTGTTGAAGCAGGAAACC2150680.2728264129072006No Hit
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT2101510.2665889090839228No Hit
ACATGCTCTGGCCGAGCCAGTCGCTCTCTTTGATGGTCAGTGTGCTGGTC2026440.25706583786135895No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG2010030.25498413280258353No Hit
ATCTTCATGAGGTAGTCAGTCAGGACCCGGCCAGCCAGGTCCAGACGCAG1915620.2430076687807073No Hit
GGTGAATCCAGAGGCTGCACAGGACAGTCTCAGGGACCTCCCAGGCTGGA1896110.24053271048109065No Hit
AAGCGCACGGACTCCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCAC1885990.23924892893357042No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1868960.23708857322662671No Hit
TCAGGGAGAACTGGTTCTTGGACGTGTCTACTGACATGGTGACTCGACTC1856390.2354939947629578No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG1775780.22526814194224554No Hit
CAGCCCCTCATGCTGTACATGGCAGGTGTATCTCTGCTCTTCTCCAGAAG1681380.2132929464792107No Hit
CGTGTGTTGTAACCACTCTCCCATATGGCCAAACACATCCAGTTTGCTAG1644960.2086728551787475No Hit
GATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGG1627790.2064947396480239No Hit
GCTTCTGAGGCTGTCTCCTTGGCAGGTGATCCTGACTGTCTGTCCCAAGG1577080.20006187776316695No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT1483740.18822114953732297No Hit
ATGTCTCGATCCCACTTAACTATCCTGGGCTGTGACAAAGTCACATGGTT1456130.18471865857615358No Hit
CTGACCTCAAGTGATCCGCCCGCCGCGGCCTCCCAAAGTGCTGGGATTGT1453250.18435331362982368No Hit
CCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGA1411840.1791002114674903No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC1389880.17631445625172498No Hit
GGCTCCATCCTCTGGCTCGCGGCGACGCTGTCGAACCGCACGAACTGCGT1387330.1759909737471621No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1380390.1751105939112144No Hit
CTGACCTCAAGTGATCCGCCCGCCCCGGCCTCCCAAAGTGCTGGGATTGT1361550.17272062905397315No Hit
GTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCAC1350550.17132521432840764No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC1336360.16952512933242814No Hit
AAGCGAGGCGCACGAGTACGAGCGAAGTCTGGTCTGCGCAGTGGCCACCA1325570.1681563543425325No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1318400.1672467976532321No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA1200310.15226638629486577No Hit
ATGTCTCGATCCCACTTAACTATCATGGGCTGTGACAAAGTCACATGGTT1198720.1520646854390795No Hit
GGCTCCATCCTCTGGCTCGCGGCGGCGCTGTCGAACCGCACGAACTGCGT1148260.14566353752525812No Hit
GCACTGGGATGACGATGAGCAGAATGGTCATGAGGATGCCCAGAATCAGG1109920.14079988292724166No Hit
GGTCTTTCAAGGATTGTAGGTGGATAGTCCCTTGGTTGGTGCTGATGCAG1094500.1388437651937671No Hit
CCTCAGCCTGGAGCCCAGAGACGGACAGGGAGGCCGTGTTGCCAGACTTG1072280.1360250274481248No Hit
TGGCCTCTCTGGGATAGAAGTTATACAGCAGGCACACAACAGAGGCAGTT1065880.13521314978961396No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG1036070.13143157588333146No Hit
TGTGGGACTTCCACTGCTCAGGCGGCAGGCTCAGATAGCTGCTGGCCGCG1027470.1303406152797075No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT994820.12619877066246085No Hit
AGTGCATGCCATAGCTACTGAAGGTGAATCCAGACGCTGCACAGGAGAGT989900.12557463971248062No Hit
ATGTAGGCTGTGTCTGTAGATGTGACCTCGGTCATGGTGACTCTGCCCTG958250.12155965097937628No Hit
CAGCAGGAGCCCCAGGAGCTGAGCGGGGACCATCATGTCCATGCTGTGTC955960.12126915100469035No Hit
AAACCTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCCCCAGACTGTACC950600.12058920346568754No Hit
TAGGAAATTTCACTCCGCTTCTCCAGCTCAATGGTCTCTTTGGTCGGCAG927440.11765122118895144No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA909060.11531961003841565No Hit
TAGTTGGAGCGCTTTGTCATGATTTCCAGGTTGGCTTTGTCCACAGCTAT900180.11419312978723185No Hit
GTCACCAAGTGGGGTTTTGAGCTCCACTCTCTTGTCCACCTTGGTGTTGC895560.11360705560249434No Hit
TGTGACTCGTGCAGCCATCGACAGAGACGCTGTAGGTGAAGCGGCTGTTG885110.11228141161320712No Hit
TTGTTCTCATCCAGCTCGCACACCATGCCGTGTTTGCAGTGGTGGTTCTG873780.11084413444587467No Hit
AGGGACGCCATCTCTGCCAGGTTCACCAGCAGCTCCTCTGGGCCCTGCAG858920.10895905600751982No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC835750.1060198051719423No Hit
CTGCTGTCTGCTGATTGACAGTAAAAGTCAGCCTCGTCTTCTGCCTGGAC823580.1044759690619303No Hit
GCTTCTGAGGCTGTCTCCTTGGCAAGTGATCCTGACTGTCTGTCCCAAGG792680.10055612224193268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)