FastQCFastQC Report
Sun 12 May 2024
EGAF00006139853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99124588
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT12196301.2304010786909905No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT9949831.0037701241189523No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT9437820.9521169459993114No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA7541450.7608051798409492No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC6795080.6855090282947759No Hit
TTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCT6392020.6448470686203508No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG6271360.6326745085689536No Hit
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG4812690.4855192941634219No Hit
CCAGTAATCTGCCCAGGGCCTCACCACCAACTGCATCCACGTTCACTTTG4391200.4429980581609076No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC3907940.39424527040657154No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT3716740.3749564134380059No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG3392080.3422036921858379No Hit
TCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGATTC3098580.3125944896739445No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA3083500.3110731718753777No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG2951670.2977737471150952No Hit
ACATAGGGACAGTTGTCCAGATTGTTCTGGTGGCCATCTTCATCAATATC2940620.2966589883833868No Hit
ATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAAAGGGTGTAACGCAA2725720.27497920092237865No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG2566590.25892566635434594No Hit
GAATAATTCTGACGAATCTGGGAGCTCATGGTTGGTTGGCAAGAAGGAGC2379740.24007565105844375No Hit
ACTCGTGAAGTCAGCCTGAAACTGTCTGAACATGTCGGTCATTCCCAGGT2104260.21228436278595175No Hit
TGTAGGAGTCGGTGGCCAGGAGGATGTTGAACTCGGCTTCTGCCGCAGGA1893960.19106863778339234No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC1806370.1822322832756692No Hit
GCCCATTCACGTAAAAACTTGACCGGTCTTTGCCAACCAGGACACCCACC1720160.17353514750548066No Hit
TCACACACAGACTTCAGGTAGGGATACTGGTCCTTTGTACTGAGATAGGC1620810.16351240723441895No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1606410.16205968997318806No Hit
GGGTCCACCTTCCCAGGGAAACCATGGCTTCAGCAACCAGTTTGTTCTCC1497460.15106847152797245No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC1464010.1476939303899049No Hit
TGATCTATCCATAGGCCTGGAAGATCAGCACTGGGATGACGATGAGCAGA1463580.14765055063835422No Hit
GGAAACGGAACACTGGATCCGCGAGAAGAGCTCGGTCCTTCCGGACGCCG1440510.14532317652609059No Hit
TCATCCCTCAGCCAGAGATTAGTCTACTTCTTCCATGCGTGATGTGTCGT1307820.1319369922627068No Hit
TCCTCAGAAAGTGGGAAGATACGATTCAAGTCCTGGATTCTCGTCTTTGG1295180.13066182933340414No Hit
GATGCCGACCACCGTGCCCACGTCCTCCTTCTTGTCCTCCTCCTCGGCCC1270860.1282083512922142No Hit
TTCTTTGATGTGAAAGGGGCAGCACAGTCATTTAAACTTGATCCAACCTC1249320.1260353283889563No Hit
GTGTGACCACACTTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAAGGCG1231020.1241891668694754No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG1207380.12180428936562138No Hit
TATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCTGAATCTCATC1195940.12065018620808796No Hit
ATTGATCTTCTCGGTCTTGCATCATACTCCTGTAGGCGGTGTCTGTGATG1140160.11502292448368108No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1128010.11379719429451753No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG1121950.11318584244708287No Hit
TTGTCCTCCTTTTCAGAATACTTTTCAGATGCAGCCTCCAGAGATTTCAG1114090.11239290094199435No Hit
GCTTCCTGCGTCTCAAAGTGTACAAATCCATAGCCCTTGGAACCATTTTC1098900.11086048599768203No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG1070140.10795908680094589No Hit
AACCGCAGTACTTGGGCCGGTATTTCTTCACACTCAAACATCCAGCGTAA1063660.107305364033392No Hit
AGCAAAGTCCTTGAGTCCCAACAGTCCACCTCAAAGACAAACATACTAAA1017450.10264355398884482No Hit
TGCTACATCTACACTTGGTTGGCTTAATCAACCTCTTCAATGGTGGGCCC1015620.10245893783689672No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG994410.10031920637087542No Hit
GGAGCTGAGCGAGGCGCGGGAGGCTGTGGCTGGGAGCGGCGCTGAGAGCT992130.10008919280451384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)