FastQCFastQC Report
Wed 8 May 2024
EGAF00006139855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14450606
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT2528281.7496013661987602No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG2233991.5459490072596263No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1892341.3095229362699392No Hit
AGGGACGCCATCTCTGCCAGGTTCTCCAGCAGCTCCTCTGGGCCCTGCAG1841261.2741749377154148No Hit
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT1721231.1911126772122913No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG1323500.9158785451627427No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT1242100.859548727575854No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG1134350.7849843805858384No Hit
CCAGGCTCCCCAGGGCGACCAGCATCTCCATTAGGTCCTCGGACTCCAGC1120290.7752546848208304No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC1020780.7063925208396105No Hit
TGTGACTCGTGCAGCCATCGACAGTGACGCTGTAGGTGAAGCGGCTGTTG954310.6603944498936585No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG952520.6591557475167477No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA911580.6308247557230472No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT815150.564093990245115No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG789090.5460601444672978No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC631120.436742929673676No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG602020.41660536589261377No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT580490.401706336744632No Hit
TGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGTCACATCCTGGC518130.3585524371780671No Hit
ACATGCTCTGGCCGAGCCAGTCGCTCTCTTTGATGGTCAGTGTGCTGGTC463970.32107304012025517No Hit
CCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGA460840.31890704099191414No Hit
ATGGCCTGCACCTCGCCGCGGTACTGCACCAGGCGGCCGCACACGTCCTC459330.3178621021153023No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC450880.3120145964812825No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC445880.30855453397594534No Hit
GATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACACTTACAT435330.301253802089684No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG426450.29510873108020524No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG426210.294942648079949No Hit
AAGCGAGGCGCACGAGTACGAGCGAAGTCTGGTCTGCGCAGTGGCCACCA422750.2925482848262557No Hit
CATTCAGGATCGCAGACCAGCTCATCAGCGAGGCAGCCACCGAGTTGGCC422220.29218151820069No Hit
TCCATGCCCAGTAGTGAGTTCTTGTCGGTGGCCTGCAGGAAGACGTTCTC419490.29029232407277594No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC403720.27937928693094255No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG400090.27686728155206775No Hit
GGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCTCCCAGGCTGGA391460.2708952136678559No Hit
GGGTTCTCCCTTCTGCCCTTTGGGTCCTCGGATCCCGGGAATGGCTGGGA389880.26980183391616935No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC373020.2581345031481725No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC372860.2580237811480017No Hit
CTTCATGGCTGTGAAATTCATGGCTGTGGAATTCACGGCTGACTTTGGAA365580.2529859301402308No Hit
CAGCCCATCATTTCCAGGAGGACATGGCATTTCTCCAGGTGGACCCATGG358270.24792731875742788No Hit
GATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGG354200.24511082787808344No Hit
GAGAGTCTCAGGGATCCCCCAGGCTGTACCAAGCCTCCCCCAGACTCCAC350060.2422458961236643No Hit
CGTGTGTTGTAACCACTCTCCCATTTGGCCAAACACATCCAGTTTGCTAG340010.23529117048793663No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA333890.23105605398140394No Hit
GGTCAATCCGGAGAGTCCAGTTGCTCCCAGTGACTGCAGAGTAGCCACAA332000.2297481503543865No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC313920.21723656433508742No Hit
CTTCGCACGCAGCACCAGAGGCTGCCATCAAGATGAGGGTGAGGGCCAGA310450.2148352809563834No Hit
GCACTGGGATGACGATGAGCAGAATGGTCATGAGGATGCCCAGAATCAGG307680.21291840632842665No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT295390.20441357269030794No Hit
CACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACA291500.20172164406115564No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT278690.19285696392248186No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT274630.19004739316814812No Hit
TATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCTGAATCTCATC272030.18824816066537278No Hit
TTTGTGAAACTTGTTTCCTGCAGGTGATGGTTCATGACAATATCGACACC266020.18408916553395754No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT259630.17966720565213667No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG253910.17570889414603097No Hit
GGTTTGCACCGCCCCTGTGTCATATTCCGGCGCCTCATCATTTGGTTACA250320.1732245692671989No Hit
TGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCT248760.1721450297655337No Hit
GCTTTCAGGGCAGTTACATTCTGGTGCACAGTTTGGTGATGATTGCCCAT244210.1689963728856769No Hit
CAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAG242710.16795835413407578No Hit
TCCGGTGGTATCTGGGAGCCAGAGTAGCAGGAGGAAGAGAAGCTGTGCTG241500.16712101900778417No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG237170.16412460487816222No Hit
ACCTGCTCTTGTCCACGGTGAGCCTGCTGTAGAGGAAGAAGGAGCCGTCG237060.16404848350304477No Hit
AAACCTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCCCCAGACTGTACC223390.15458867261345302No Hit
TAGTTGGAGCGCTTTGTCATGATTTCCAGGTTGGCTTTGTCCACAGCTAT220760.15276867973564567No Hit
TGGTGCAGACCACACCAGCGTCTCTCTCGTGCCTGCAGTTGCTCTTCAGC216820.15004215048144No Hit
GTGCACAGGAGCAGAGGGCCATGGTGCAGACGAGGACAAGGAGGGCAGCT215850.14937089835540462No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC209430.1449281780985517No Hit
CATGCTGGAGATTGTCCTGAGTCCTGTCTTCTCTACCCACAGCTGAAGCT209300.14483821647341297No Hit
GTGATGTTGGCGGTTAGTGGGGTCTTCAACTCGGGGTGGGCAGCAGTGCA208690.14441608884776183No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC205430.142160128094282No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA199730.13821565683819764No Hit
ACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCCACCCAT198330.13724683933670326No Hit
GTGCTTCATAGTAGAGGGTCACATTGACAAGCGGTGCATTGGACTTCTTC197240.13649254571053976No Hit
GGTGCTCCGCCTCAGAGGCATCATTTGACTTTTGCTCGGAGATCTGAGCC191180.13229894995407113No Hit
GGCTCCACCTCAAGGGTGATGGTCTTGCCGGTAAGGGTTTTCACGAAGAT187290.12960702132491883No Hit
AGGAGCTGCAGAGGGTCAGGATGCTCAGGCAGAAGTCCAGCAGCTGCAGC186770.12924717482436376No Hit
CCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTG182620.12637532294493395No Hit
GTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCAC181970.1259255148192401No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA180910.12519198156810862No Hit
CAGCAGGAGCCCCAGGAGCTGAGCGGGGACCATCATGTCCATGCTGTGTC178420.12346887044045073No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC168840.11683939068022477No Hit
CTGACTCCCCAAAGACATGCCTTCTCTCTGAGTGCCCCAGGACCACCCAC166040.11490175567723596No Hit
TCCCACCGGTGGACTTACGAGCAGTCTGCTTGGTTCGGGCCATTTTCTTT164490.11382913630058145No Hit
TGCAAATCCAGCAAGAAGTCCCAGTCTTTTCTGTTCAGTTTCATGGCTAT164030.11351081055009044No Hit
CCTGCACACCACACCCACAGTGGTTTCAGACATGCTACTCTTGCCAACAG163490.113137123799514No Hit
CAGCACCAGTTCAAGAAGAGGGTGTTGAACAGGGACCAGACGACATGGTC162560.1124935521735213No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG155730.10776710679123076No Hit
CGGTGACCACAGCTCCAAGGACAGCTAGGACAACCAGGACAGCCAGGCCA155550.10764254454103864No Hit
GGCGTTGTCTCTGGAGATGGTGAATCGGCCCTTCACTGAGTCTGCGTAGT152580.10558726741286836No Hit
AGCCTCGATGCACCCTGAGCCAGGTGTTCTCCAGGGTGTGCTGACTGCAG152510.10553882653779365No Hit
AGCTCCAGGATCTCGGCGGTCAGGTACTCGAGGACCGCCGCCATGTAGAC152170.1053035422874307No Hit
CCATCAGGAGTCTGCCAGGTGACTTCACACGTGTAGTGGCTCCGGTCATC150700.1042862839108616No Hit
CGGCTGCGCTTGCGCTTCTTGCCATCTTTCTTCTGCGCTTTGGTCACTGC149150.10321366453420706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146810.10159435528170929No Hit
AAGCGCACGGACTCCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCAC145770.10087466228059917No Hit
TTAAACCCATTAACATGCAGGATCCTCATCTGCTTCACAATGGTGCTTTT145730.10084698178055647No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)