FastQCFastQC Report
Sun 12 May 2024
EGAF00006139861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89725125
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCTCGATCCCACTTAACTATCGTGGGCTGTGACAAAGTCACATGGTT4047360.45108435346286785No Hit
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG3633640.40497463781744525No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC3324800.3705539557621123No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT3228950.3598713292402769No Hit
GCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGG2738510.30521105431728296No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG2387930.2661383865444601No Hit
TGGGCTCAACTTTCTTGTCCACCTGGGTGTTGCTGGGCTTGTGATTCACG2290890.25532313273456014No Hit
GGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCTCCCAGGCTGGA2229470.24847778144638974No Hit
GTGTAGGAGGCCTGCAGGTACAAAATGACCAGGCTGTTGACGGCTGCCTC2202160.24543404091106033No Hit
ACATGCTCTGGCCGAGCCAGTCGCTCTCTTTGATGGTCAGTGTGCTGGTC2121130.23640312565738972No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC2067120.23038363000330175No Hit
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT2056380.2291866408656438No Hit
CTGCCCACTCAGACTTTATTCAAACACCAGGAAGGGCCGGTGCAAGGAGG1886140.21021313706723732No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG1852050.20641375534444784No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG1844310.2055511207145156No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG1813260.2020905515595548No Hit
GATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACACTTACAT1799890.20060044497012403No Hit
AAGCGCACGGACTCCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCAC1792770.1998069102717884No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG1790950.19960406853710153No Hit
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT1783250.19874589196727227No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1748870.194914189308736No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT1747780.194792707170929No Hit
AACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCT1709990.1905809548886112No Hit
CTGACCTCAAGTGATCCGCCCGCCACGGCCTCCCAAAGTGCTGGGATTGT1610800.1795260803481745No Hit
TCAGGGAGAACTGGTTCTTGGACGTGTCTACTGACATGGTGACTCGACTC1604830.1788607148778004No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT1548500.1725826517377379No Hit
ATGTCTCGATCCCACTTAACTATCCTGGGCTGTGACAAAGTCACATGGTT1451000.16171613023665332No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG1401830.15623605985502947No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT1391360.15506916262306683No Hit
CCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGA1364130.15203433820794343No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC1310320.1460371328543705No Hit
GGTGAATCCAGAGGCTGCACAGGACAGTCTCAGGGACCTCCCAGGCTGGA1307660.1457406718575204No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1306440.14560470102437864No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1219340.1358972751500764No Hit
TGGGCTCAACTTTCTTGTCCACCTCGGTGTTGCTGGGCTTGTGATTCACG1140720.12713495801761213No Hit
ATGTCTCGATCCCACTTAACTATCATGGGCTGTGACAAAGTCACATGGTT1122900.1251488922417216No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA1077840.1201268875356819No Hit
CGTGTGTTGTAACCACTCTCCCATATGGCCAAACACATCCAGTTTGCTAG1074450.11974906694195188No Hit
CCCGCCTCTTCACGGGTAGGTCAATTTCACTGGTTGAAAGTAAGAGACAG1062350.11840050376079164No Hit
GGCAGCTGTAGCTTTTGTGGGACTACCACTGCTCAGGCGTCAGGCTCAGG1049780.11699955837342106No Hit
CAGCCCCTCATGCTGTACATGGCAGGTGTATCTCTGCTCTTCTCCAGAAG1043840.1163375364481242No Hit
GCTTCTGAGGCTGTCTCCTTGGCAGGTGATCCTGACTGTCTGTCCCAAGG981020.10933615305634847No Hit
GGTCTTTCAAGGATTGTAGGTGGATAGTCCCTTGGTTGGTGCTGATGCAG978370.10904080657452414No Hit
TGTGGGACTTCCACTGCTCAGGCGACAGGCTCAGATAGCTGCTGGCCGCG956700.10662565251371899No Hit
AAACCTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCCCCAGACTGTACC942810.1050775911429491No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT932790.10396084708714531No Hit
TGGGCTCAACTTTCTTGTCCACCTAGGTGTTGCTGGGCTTGTGATTCACG914630.10193688780037921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)