FastQCFastQC Report
Sun 12 May 2024
EGAF00006139865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91780970
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCTCGATCCCACTTAACTATCGTGGGCTGTGACAAAGTCACATGGTT4047860.44103478095731613No Hit
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG3790420.41298539337729817No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC3445340.37538718538276505No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT3384980.36881065868011637No Hit
GCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGG2840540.30949117229857126No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG2492760.27159878567419804No Hit
GGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCTCCCAGGCTGGA2298060.25038523781128047No Hit
TGGGCTCAACTTTCTTGTCCACCTGGGTGTTGCTGGGCTTGTGATTCACG2284910.24895247892891087No Hit
GTGTAGGAGGCCTGCAGGTACAAAATGACCAGGCTGTTGACGGCTGCCTC2244340.24453217262794236No Hit
ACATGCTCTGGCCGAGCCAGTCGCTCTCTTTGATGGTCAGTGTGCTGGTC2174220.23689224465594555No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC2169880.23641937974723956No Hit
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT2169570.23638560368233197No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG1992530.2170962019686652No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG1947420.21218123974937286No Hit
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT1886610.20555568327508414No Hit
CTGCCCACTCAGACTTTATTCAAACACCAGGAAGGGCCGGTGCAAGGAGG1876720.2044781178494845No Hit
AAGCGCACGGACTCCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCAC1863070.20299088144307037No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1862700.2029505680752775No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG1844860.2010068100173707No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG1836990.2001493337889107No Hit
GATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACACTTACAT1834930.19992488638984748No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT1797040.19579657961775737No Hit
AACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCT1751860.19087399054509882No Hit
TCAGGGAGAACTGGTTCTTGGACGTGTCTACTGACATGGTGACTCGACTC1665940.1815125728132967No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT1662150.1810996331810396No Hit
CTGACCTCAAGTGATCCGCCCGCCACGGCCTCCCAAAGTGCTGGGATTGT1634720.17811099621195986No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG1459660.15903732549350916No Hit
ATGTCTCGATCCCACTTAACTATCCTGGGCTGTGACAAAGTCACATGGTT1448060.1577734469356774No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT1442440.15716111956541753No Hit
CCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGA1408140.15342396141596673No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC1380390.1504004588314985No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1359100.14808080585768488No Hit
GGTGAATCCAGAGGCTGCACAGGACAGTCTCAGGGACCTCCCAGGCTGGA1323080.1441562450255211No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1284300.1399309682606318No Hit
ATGTCTCGATCCCACTTAACTATCATGGGCTGTGACAAAGTCACATGGTT1195340.13023832718263928No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA1129550.1230701745688676No Hit
CGTGTGTTGTAACCACTCTCCCATATGGCCAAACACATCCAGTTTGCTAG1107620.12068079036427705No Hit
TGGGCTCAACTTTCTTGTCCACCTCGGTGTTGCTGGGCTTGTGATTCACG1103690.12025259702528748No Hit
CCCGCCTCTTCACGGGTAGGTCAATTTCACTGGTTGAAAGTAAGAGACAG1100580.11991374682573086No Hit
GGCAGCTGTAGCTTTTGTGGGACTACCACTGCTCAGGCGTCAGGCTCAGG1033600.11261593770473335No Hit
CAGCCCCTCATGCTGTACATGGCAGGTGTATCTCTGCTCTTCTCCAGAAG1023330.11149696936085988No Hit
GGTCTTTCAAGGATTGTAGGTGGATAGTCCCTTGGTTGGTGCTGATGCAG1012590.11032679214438461No Hit
AAACCTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCCCCAGACTGTACC1010640.11011432980061117No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT980200.1067977381367837No Hit
TGGGCTCAACTTTCTTGTCCACCTAGGTGTTGCTGGGCTTGTGATTCACG957760.10435278685766776No Hit
GCTTCTGAGGCTGTCTCCTTGGCAGGTGATCCTGACTGTCTGTCCCAAGG955170.10407059328311741No Hit
TGTGGGACTTCCACTGCTCAGGCGACAGGCTCAGATAGCTGCTGGCCGCG949130.10341250479266018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)